Product overview
Full product name
Size
Sensitivity
Detection range
Sample type
Code
Assay type
Storage
Assay time
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated Plate | 8 wells * 12 detachable strips |
| Standard, lyophilized | 2 vials |
| Standard/Sample Diluent | 6ml ×1 vial |
| Biotinylated Antigen, lyophilized | 1 vial |
| Avidin-HRP Concentrate | 100μl × 1 vial |
| Biotinylated Antigen Diluent | 6ml × 1 vials |
| Avidin HRP Diluent | 5.9ml × 1 vials |
| Substrate Solution A | 6ml ×1 vial |
| Substrate Solution B | 6ml ×1 vial |
| Stop Solution | 6ml ×1 vial |
| Wash Buffer Concentrate (25x) | 20ml × 1 vial |
Protocol data
These standard curves of EA0240Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 0pg/ml | 2.086 | 2.125 | 2.09 |
| 2.165 | |||
| 15pg/ml | 1.676 | 1.711 | 1.676 |
| 1.746 | |||
| 30pg/ml | 1.164 | 1.182 | 1.147 |
| 1.2 | |||
| 60pg/ml | 0.691 | 0.668 | 0.633 |
| 0.644 | |||
| 120pg/ml | 0.418 | 0.403 | 0.368 |
| 0.388 | |||
| 240pg/ml | 0.174 | 0.189 | 0.154 |
| 0.205 |
Precision
We measured random samples of EA0240Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-assay | 1 | 18 | 19.7 | 1.2 | 6.2 |
| Intra-assay | 2 | 18 | 112.1 | 5.5 | 4.9 |
| Intra-assay | 3 | 18 | 41.9 | 2.5 | 6.1 |
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