Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Storage
Assay time
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Standard Solution | 0.5ml x1 |
| Pre-coated ELISA Plate | 12 wells * 8 detachable strips |
| Standard Diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop Solution | 6ml x1 |
| Substrate Solution A | 6ml x1 |
| Substrate Solution B | 6ml x1 |
| Wash Buffer Concentrate (25x) | 20ml x1 |
| Biotinylated Antibody | 1ml x1 |
Protocol data
These standard curves of E1827Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 24ng/ml | 1.969 | 1.904 | 1.822 |
| 1.839 | |||
| 12ng/ml | 1.192 | 1.178 | 1.096 |
| 1.163 | |||
| 6ng/ml | 0.559 | 0.578 | 0.496 |
| 0.598 | |||
| 3ng/ml | 0.289 | 0.303 | 0.221 |
| 0.316 | |||
| 1.5ng/ml | 0.163 | 0.158 | 0.076 |
| 0.152 | |||
| 0ng/ml | 0.086 | 0.082 | 0 |
| 0.078 |
Precision
We measured random samples of E1827Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-assay | 1 | 18 | 8 | 0.3 | 3.7 |
| Intra-assay | 2 | 18 | 19 | 0.7 | 3.6 |
| Intra-assay | 3 | 18 | 6.1 | 0.3 | 5.1 |
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