Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Storage
Assay time
UniProt accession
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Standard Solution | 0.5ml x1 |
| Pre-coated ELISA Plate | 12 wells * 8 detachable strips |
| Standard Diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop Solution | 6ml x1 |
| Substrate Solution A | 6ml x1 |
| Substrate Solution B | 6ml x1 |
| Wash Buffer Concentrate (25x) | 20ml x1 |
| Biotinylated Antibody | 1ml x1 |
Protocol data
These standard curves of E1558Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 8ng/ml | 1.945 | 1.999 | 1.935 |
| 2.053 | |||
| 4ng/ml | 1.078 | 1.115 | 1.051 |
| 1.153 | |||
| 2ng/ml | 0.594 | 0.607 | 0.543 |
| 0.62 | |||
| 1ng/ml | 0.325 | 0.341 | 0.277 |
| 0.357 | |||
| 0.5ng/ml | 0.157 | 0.162 | 0.098 |
| 0.166 | |||
| 0ng/ml | 0.067 | 0.064 | 0 |
| 0.06 |
Precision
We measured random samples of E1558Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-assay | 1 | 18 | 3.3 | 0.2 | 5.1 |
| Intra-assay | 2 | 18 | 5.9 | 0.2 | 3.3 |
| Intra-assay | 3 | 18 | 2.5 | 0.2 | 6.2 |
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