Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Storage
Assay time
UniProt accession
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Standard Solution | 0.5ml x1 |
| Pre-coated ELISA Plate | 12 wells * 8 detachable strips |
| Standard Diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop Solution | 6ml x1 |
| Substrate Solution A | 6ml x1 |
| Substrate Solution B | 6ml x1 |
| Wash Buffer Concentrate (25x) | 20ml x1 |
| Biotinylated Antibody | 1ml x1 |
Protocol data
These standard curves of E1830Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 24ng/ml | 2.167 | 2.195 | 2.111 |
| 2.222 | |||
| 12ng/ml | 1.172 | 1.213 | 1.129 |
| 1.254 | |||
| 6ng/ml | 0.611 | 0.594 | 0.51 |
| 0.577 | |||
| 3ng/ml | 0.322 | 0.334 | 0.25 |
| 0.346 | |||
| 1.5ng/ml | 0.167 | 0.159 | 0.075 |
| 0.15 | |||
| 0ng/ml | 0.081 | 0.084 | 0 |
| 0.086 |
Precision
We measured random samples of E1830Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-assay | 1 | 18 | 11 | 0.5 | 4.8 |
| Intra-assay | 2 | 18 | 18.7 | 0.6 | 3 |
| Intra-assay | 3 | 18 | 4.8 | 0.2 | 4.4 |
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