Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Storage
Assay time
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Standard Solution | 0.5ml x1 |
| Pre-coated ELISA Plate | 12 wells * 8 detachable strips |
| Standard Diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop Solution | 6ml x1 |
| Substrate Solution A | 6ml x1 |
| Substrate Solution B | 6ml x1 |
| Wash Buffer Concentrate (25x) | 20ml x1 |
| Biotinylated Antibody | 1ml x1 |
Protocol data
These standard curves of E3451Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 2400ng/L | 2.189 | 2.249 | 2.178 |
| 2.308 | |||
| 1200ng/L | 1.088 | 1.055 | 0.984 |
| 1.021 | |||
| 600ng/L | 0.6 | 0.613 | 0.542 |
| 0.626 | |||
| 300ng/L | 0.304 | 0.299 | 0.228 |
| 0.294 | |||
| 150ng/L | 0.156 | 0.151 | 0.08 |
| 0.147 | |||
| 0ng/L | 0.068 | 0.071 | 0 |
| 0.074 |
Precision
We measured random samples of E3451Hu within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-assay | 1 | 18 | 1181.6 | 60.1 | 5.1 |
| Intra-assay | 2 | 18 | 1553 | 33.5 | 2.2 |
| Intra-assay | 3 | 18 | 478.3 | 22.3 | 4.7 |
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