Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Standard Solution | 0.5ml x1 |
| Pre-coated ELISA Plate | 12 wells * 8 detachable strips |
| Standard Diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop Solution | 6ml x1 |
| Substrate Solution A | 6ml x1 |
| Substrate Solution B | 6ml x1 |
| Wash Buffer Concentrate (25x) | 20ml x1 |
| Biotinylated Antibody | 1ml x1 |
Protocol data
These standard curves of E0023FI are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 64KU/L | 1.963 | 1.979 | 1.896 |
| 1.995 | |||
| 32KU/L | 1.052 | 1.088 | 1.004 |
| 1.123 | |||
| 16KU/L | 0.553 | 0.574 | 0.49 |
| 0.595 | |||
| 8KU/L | 0.292 | 0.28 | 0.197 |
| 0.269 | |||
| 4KU/L | 0.175 | 0.167 | 0.083 |
| 0.158 | |||
| 0KU/L | 0.079 | 0.084 | 0 |
| 0.088 |
Precision
We measured random samples of E0023FI within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-assay | 1 | 18 | 49.9 | 1.3 | 2.7 |
| Intra-assay | 2 | 18 | 29.1 | 1.1 | 3.7 |
| Intra-assay | 3 | 18 | 22.3 | 1.3 | 6 |
Customer reviews and Q&As