Full product name

Code

Assay type

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Detection range

Sample type

Species

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Assay time

Gene names

Research area

Target protein

Components	Quantity
Standard Solution	0.5ml x1
Pre-coated ELISA Plate	12 wells * 8 detachable strips
Standard Diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop Solution	6ml x1
Substrate Solution A	6ml x1
Substrate Solution B	6ml x1
Wash Buffer Concentrate (25x)	20ml x1
Biotinylated Antibody	1ml x1

These standard curves of E0061FI are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
160ng/ml	1.984	1.973	1.89
	1.961
80ng/ml	1.057	1.074	0.991
	1.09
40ng/ml	0.572	0.597	0.515
	0.623
20ng/ml	0.321	0.331	0.248
	0.341
10ng/ml	0.163	0.156	0.073
	0.149
0ng/ml	0.088	0.083	0
	0.077

We measured random samples of E0061FI within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	78	4	5.2
Intra-assay	2	18	110.8	3.2	2.9
Intra-assay	3	18	56.2	3.1	5.6

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