Product overview
Full product name
Size
Sensitivity
Detection range
Sample type
Code
Assay type
Species
Storage
Assay time
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated Plate | 8 wells * 12 detachable strips |
| Standard, lyophilized | 2 vials |
| Standard/Sample Diluent | 6ml ×1 vial |
| Biotinylated Antigen, lyophilized | 1 vial |
| Avidin-HRP Concentrate | 100μl × 1 vial |
| Biotinylated Antigen Diluent | 6ml × 1 vials |
| Avidin HRP Diluent | 5.9ml × 1 vials |
| Substrate Solution A | 6ml ×1 vial |
| Substrate Solution B | 6ml ×1 vial |
| Stop Solution | 6ml ×1 vial |
| Wash Buffer Concentrate (25x) | 20ml × 1 vial |
Protocol data
These standard curves of EA0022FI are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 0ng/L | 2.041 | 2.088 | 2.055 |
| 2.135 | |||
| 80ng/L | 1.665 | 1.704 | 1.671 |
| 1.743 | |||
| 160ng/L | 1.153 | 1.11 | 1.077 |
| 1.066 | |||
| 320ng/L | 0.724 | 0.736 | 0.703 |
| 0.749 | |||
| 640ng/L | 0.364 | 0.381 | 0.348 |
| 0.398 | |||
| 1280ng/L | 0.222 | 0.213 | 0.18 |
| 0.204 |
Precision
We measured random samples of EA0022FI within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-assay | 1 | 18 | 151.8 | 11.5 | 7.6 |
| Intra-assay | 2 | 18 | 80.6 | 4.6 | 5.8 |
| Intra-assay | 3 | 18 | 359.6 | 20 | 5.6 |
Customer reviews and Q&As