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Home > Product > ELISA Kit > Fish Insulin-like growth factor 1, IGF-1 ELISA Kit

Fish Insulin-like growth factor 1, IGF-1 ELISA Kit

Fish Insulin-like growth factor 1, IGF-1 ELISA Kit

Product Summary

Size:

Sensitivity: 5.38 ng/L

Detection range: 10-2000 ng/L

Sample type: Serum, plasma, cell culture supernates, ascites, tissue homogenates or other biological fluids

Reactive with: Fish

Product Summary

Size:

Sensitivity: 5.38 ng/L

Detection range: 10-2000 ng/L

Sample type: Serum, plasma, cell culture supernates, ascites, tissue homogenates or other biological fluids

Reactive with: Fish

Product overview

Full product name

Insulin-like growth factor 1

Size

Sensitivity

5.38 ng/L

Detection range

10-2000 ng/L

Sample type

Serum, plasma, cell culture supernates, ascites, tissue homogenates or other biological fluids

Code

EA0022FI

Assay type

Competitive

Species

Fish

Storage

2-8°C

Assay time

2h 30m

Research area

Signal Transduction

Target protein

IGF-1

Components

Components Quantity
Pre-coated Plate 8 wells * 12 detachable strips
Standard, lyophilized 2 vials
Standard/Sample Diluent 6ml ×1 vial
Biotinylated Antigen, lyophilized 1 vial
Avidin-HRP Concentrate 100μl × 1 vial
Biotinylated Antigen Diluent 6ml × 1 vials
Avidin HRP Diluent 5.9ml × 1 vials
Substrate Solution A 6ml ×1 vial
Substrate Solution B 6ml ×1 vial
Stop Solution 6ml ×1 vial
Wash Buffer Concentrate (25x) 20ml × 1 vial

Protocol data

These standard curves of EA0022FI are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
0ng/L 2.041 2.088 2.055
2.135
80ng/L 1.665 1.704 1.671
1.743
160ng/L 1.153 1.11 1.077
1.066
320ng/L 0.724 0.736 0.703
0.749
640ng/L 0.364 0.381 0.348
0.398
1280ng/L 0.222 0.213 0.18
0.204

Precision

We measured random samples of EA0022FI within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 18 151.8 11.5 7.6
Intra-assay 2 18 80.6 4.6 5.8
Intra-assay 3 18 359.6 20 5.6

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Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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