Product overview
Full product name
Size
Sensitivity
Detection range
Sample type
Code
Assay type
Species
Storage
Assay time
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated Plate | 8 wells * 12 detachable strips |
| Standard, lyophilized | 2 vials |
| Standard/Sample Diluent | 6ml ×1 vial |
| Biotinylated Antigen, lyophilized | 1 vial |
| Avidin-HRP Concentrate | 100μl × 1 vial |
| Biotinylated Antigen Diluent | 6ml × 1 vials |
| Avidin HRP Diluent | 5.9ml × 1 vials |
| Substrate Solution A | 6ml ×1 vial |
| Substrate Solution B | 6ml ×1 vial |
| Stop Solution | 6ml ×1 vial |
| Wash Buffer Concentrate (25x) | 20ml × 1 vial |
Protocol data
These standard curves of EA0007FI are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 0ng/mL | 2.179 | 2.224 | 2.186 |
| 2.269 | |||
| 1.5ng/mL | 1.63 | 1.59 | 1.552 |
| 1.549 | |||
| 3ng/mL | 1.273 | 1.297 | 1.259 |
| 1.321 | |||
| 6ng/mL | 0.692 | 0.719 | 0.681 |
| 0.746 | |||
| 12ng/mL | 0.42 | 0.399 | 0.361 |
| 0.378 | |||
| 24ng/mL | 0.215 | 0.23 | 0.192 |
| 0.245 |
Precision
We measured random samples of EA0007FI within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-assay | 1 | 18 | 1.9 | 0.1 | 6.1 |
| Intra-assay | 2 | 18 | 5.8 | 0.3 | 5.3 |
| Intra-assay | 3 | 18 | 6.5 | 0.4 | 5.9 |
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