Full product name

Size

Sensitivity

Detection range

Sample type

Code

Assay type

Species

Storage

Assay time

Research area

Target protein

Components	Quantity
Pre-coated Plate	8 wells * 12 detachable strips
Standard, lyophilized	2 vials
Standard/Sample Diluent	6ml ×1 vial
Biotinylated Antigen, lyophilized	1 vial
Avidin-HRP Concentrate	100μl × 1 vial
Biotinylated Antigen Diluent	6ml × 1 vials
Avidin HRP Diluent	5.9ml × 1 vials
Substrate Solution A	6ml ×1 vial
Substrate Solution B	6ml ×1 vial
Stop Solution	6ml ×1 vial
Wash Buffer Concentrate (25x)	20ml × 1 vial

These standard curves of EA0004FI are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
0ng/ml	2.11	2.056	2.008
	2.002
0.8ng/ml	1.526	1.493	1.445
	1.46
1.6ng/ml	1.253	1.282	1.234
	1.312
3.2ng/ml	0.723	0.765	0.717
	0.807
6.4ng/ml	0.413	0.402	0.354
	0.391
12.8ng/ml	0.184	0.173	0.125
	0.162

We measured random samples of EA0004FI within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	5	0.3	5.9
Intra-assay	2	18	2.6	0.2	7.5
Intra-assay	3	18	1.5	0.1	6.4

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