Full product name

Size

Sensitivity

Detection range

Sample type

Code

Assay type

Species

Storage

Assay time

Research area

Target protein

Components	Quantity
Pre-coated Plate	8 wells * 12 detachable strips
Standard, lyophilized	2 vials
Standard/Sample Diluent	6ml ×1 vial
Biotinylated Antigen, lyophilized	1 vial
Avidin-HRP Concentrate	100μl × 1 vial
Biotinylated Antigen Diluent	6ml × 1 vials
Avidin HRP Diluent	5.9ml × 1 vials
Substrate Solution A	6ml ×1 vial
Substrate Solution B	6ml ×1 vial
Stop Solution	6ml ×1 vial
Wash Buffer Concentrate (25x)	20ml × 1 vial

These standard curves of EA0008FI are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
0ug/ml	1.969	2.001	1.95
	2.033
3ug/ml	1.683	1.705	1.654
	1.728
6ug/ml	1.178	1.209	1.158
	1.24
12ug/ml	0.698	0.71	0.659
	0.721
24ug/ml	0.399	0.41	0.359
	0.422
48ug/ml	0.226	0.212	0.161
	0.199

We measured random samples of EA0008FI within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	18	7.2	0.4	5.6
Intra-assay	2	18	5.6	0.2	3.7
Intra-assay	3	18	11.9	0.6	4.8

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