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Home > Product > ELISA Kit > Rat Calcium/Calmodulin-dependent Protein Kinase-2, CAMK 2 ELISA Kit

Rat Calcium/Calmodulin-dependent Protein Kinase-2, CAMK 2 ELISA Kit

Rat Calcium/Calmodulin-dependent Protein Kinase-2, CAMK 2 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 5.65ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 5.65ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Calcium/Calmodulin-Dependent Protein Kinase-2

Code

E0238Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

5.65ng/L

Detection range

10-2000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade involved in a number of cellular processes. Phosphorylates CAMK1 and CAMK4. Phosphorylates CAMK1D (By similarity). Seems to be involved in hippocampal activation of CREB1 (By similarity). Efficiently phosphorylates 5'-AMP-activated protein kinase (AMPK) trimer, including that consisting of PRKAA1, PRKAB1 and PRKAG1. This phosphorylation is stimulated in response to Ca(2+) signals. May play a role in neurite growth. Isoform 2 may promote neurite elongation, while isoform 1 may promoter neurite branching.

UniProt accession

MASS(Da)

64,446

GeneID

83506

Synonyms

Calcium/calmodulin-dependent protein kinase 2;Calcium/calmodulin-dependent protein kinase beta;CaM-kinase 2;CaM-kinase beta;CAMKK 2;CaM-KK 2;CaMKK beta;CaM-KK beta;CAMKK2;

Gene names

CAMKK2

Research area

Signal Transduction

Target protein

CAMKK2

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0238Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1200ng/L 2.051 2.184 2.136
2.318
600ng/L 1.864 1.809 1.761
1.754
300ng/L 1.107 1.189 1.141
1.272
150ng/L 0.73 0.674 0.626
0.618
75ng/L 0.389 0.369 0.321
0.349
0ng/L 0.056 0.048 0
0.04

Precision

We measured random samples of E0238Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 236.2 13.78 5.8
Intra-Assay 2 18 313.9 14.04 4.5
Intra-Assay 3 18 120.8 6.12 5.1

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E0238Ra

    $320.00

  • 96T

    E0238Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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