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Home > Product > ELISA Kit > Rat Brain Derived Neurotrophic Facor, BDNF ELISA Kit

Rat Brain Derived Neurotrophic Facor, BDNF ELISA Kit

Rat Brain Derived Neurotrophic Facor, BDNF ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 0.01ng/ml

Detection range: 0.05-10ng/ml

Sample type: Serum, plasma, cell culture supernates, ascites, tissue homogenates

Reactive with: Rat

Product Summary

Size: 96T,48T

Sensitivity: 0.01ng/ml

Detection range: 0.05-10ng/ml

Sample type: Serum, plasma, cell culture supernates, ascites, tissue homogenates

Reactive with: Rat

Product overview

Full product name

Brain Derived Neurotrophic Facor

Code

E0476Ra

Assay type

Sandwich

Size

96T,48T

Sensitivity

0.01ng/ml

Detection range

0.05-10ng/ml

Sample type

Serum, plasma, cell culture supernates, ascites, tissue homogenates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Important signaling molecule that activates signaling cascades downstream of NTRK2 (By similarity). During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability (By similarity). [BDNF precursor form]: Important signaling molecule that activates signaling cascades downstream of NTRK2. Activates signaling cascades via the heterodimeric receptor formed by NGFR and SORCS2. Signaling via NGFR and SORCS2 plays a role in synaptic plasticity and long-term depression (LTD). Binding to NGFR and SORCS2 promotes neuronal apoptosis. Promotes neuronal growth cone collapse.

UniProt accession

MASS(Da)

28,109

GeneID

24225

Synonyms

Brain-derived neurotrophic factor; BDNF [Cleaved into: BDNF precursor form; ProBDNF]

Gene names

BDNF

Research area

Cardiovascular

Target protein

BDNF

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of Rat Brain Derived Neurotrophic Facor, BDNF ELISA Kit are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
6.4ng/ml 2.191 2.102 2.061
2.014
3.2ng/ml 1.334 1.333 1.292
1.332
1.6ng/ml 0.63 0.635 0.593
0.64
0.8ng/ml 0.402 0.358 0.316
0.314
0.4ng/ml 0.182 0.18 0.138
0.178
0ng/ml 0.038 0.042 0
0.045

Precision

We measured random samples of E0476Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 3.1 0.09 2.9
Intra-Assay 2 18 2.9 0.17 5.7
Intra-Assay 3 18 1.5 0.08 5.1

Protocols

References

Hippocampal BDNF content in response to short- and long-term exercise
Evaluation of brain-derived neurotrophic factor in diabetic rats

Customer reviews and Q&As

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Product size

Price

  • 96T

    $458.00

  • 48T

    $300.00

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Lead time: Within one week

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