Tel:86 021 3100 7137

Publish and get free product : share with us your publications using BT LAB product and get free products.
Home > Product > ELISA Kit > Rat Brain Derived Neurotrophic Facor, BDNF ELISA Kit

Rat Brain Derived Neurotrophic Facor, BDNF ELISA Kit

Rat Brain Derived Neurotrophic Facor, BDNF ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.01ng/ml

Detection range: 0.05-10ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 0.01ng/ml

Detection range: 0.05-10ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Brain Derived Neurotrophic Facor

Code

E0476Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.01ng/ml

Detection range

0.05-10ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Important signaling molecule that activates signaling cascades downstream of NTRK2 (By similarity). During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability (By similarity). [BDNF precursor form]: Important signaling molecule that activates signaling cascades downstream of NTRK2. Activates signaling cascades via the heterodimeric receptor formed by NGFR and SORCS2. Signaling via NGFR and SORCS2 plays a role in synaptic plasticity and long-term depression (LTD). Binding to NGFR and SORCS2 promotes neuronal apoptosis. Promotes neuronal growth cone collapse.

UniProt accession

MASS(Da)

28,109

GeneID

24225

Synonyms

BDNF;BDNF precursor form;Brain-derived neurotrophic factor;ProBDNF

Gene names

BDNF

Research area

Neuroscience; Cardiovascular; Metabolism

Target protein

BDNF

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0476Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
6.4ng/ml 2.191 2.102 2.061
2.014
3.2ng/ml 1.334 1.333 1.292
1.332
1.6ng/ml 0.63 0.635 0.593
0.64
0.8ng/ml 0.402 0.358 0.316
0.314
0.4ng/ml 0.182 0.18 0.138
0.178
0ng/ml 0.038 0.042 0
0.045

Precision

We measured random samples of E0476Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 3.1 0.09 2.9
Intra-Assay 2 18 2.9 0.17 5.7
Intra-Assay 3 18 1.5 0.08 5.1

Protocols

Customer reviews and Q&As

No reviews
No reviews
  • Size

    Code

    Price

  • 48T

    E0476Ra

    $320.00

  • 96T

    E0476Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

Newsletter

Sign up

Newsletter

Sign up