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Home > Product > ELISA Kit > Rat Nesfatin 1, NES-1 ELISA Kit

Rat Nesfatin 1, NES-1 ELISA Kit

Rat Nesfatin 1, NES-1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 16.23ng/L

Detection range: 30-9000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 16.23ng/L

Detection range: 30-9000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Nesfatin 1

Code

E0878Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

16.23ng/L

Detection range

30-9000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Calcium-binding protein which may have a role in calcium homeostasis (By similarity). Acts as a non-receptor guanine nucleotide exchange factor which binds to and activates guanine nucleotide-binding protein (G-protein) alpha subunit GNAI3 (PubMed: 21653697). [Nesfatin-1]: Anorexigenic Peptide, seems to play an important role in hypothalamic pathways regulating food intake and energy homeostasis, acting in a leptin-independent manner. May also exert hypertensive roles and modulate blood pressure through directly acting on peripheral arterial resistance.

UniProt accession

MASS(Da)

50,090

GeneID

59295

Synonyms

DNA-binding protein NEFA;Nesfatin-1;NUCB 2;NUCB2;Nucleobindin-2;Prepronesfatin

Gene names

NUCB2

Research area

Others

Target protein

NUCB2

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0878Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
4800ng/L 2.012 2.179 2.133
2.347
2400ng/L 1.527 1.492 1.446
1.456
1200ng/L 0.92 0.897 0.851
0.873
600ng/L 0.467 0.447 0.401
0.427
300ng/L 0.272 0.274 0.228
0.276
0ng/L 0.06 0.046 0
0.033

Precision

We measured random samples of E0878Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 442.9 24.26 5.5
Intra-Assay 2 18 1074.9 52.2 4.9
Intra-Assay 3 18 2045.5 68.24 3.3

Protocols

References (2)

Metabolic cross-talk between skeletal muscle and adipose tissue in high-intensity interval training vs. moderate-intensity continuous training by regulation of PGC-1α
Melatonin Treatment Affects Leptin and Nesfatin-1 Levels but not Orexin-A Levels in REM Sleep Deprived Rats
Journal:

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  • Size

    Code

    Price

  • 48T

    E0878Ra

    $320.00

  • 96T

    E0878Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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