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Home > Product > ELISA Kit > Rat Vascular Endothelial Cell Growth Factor A, VEGF-A ELISA Kit

Rat Vascular Endothelial Cell Growth Factor A, VEGF-A ELISA Kit

Rat Vascular Endothelial Cell Growth Factor A, VEGF-A ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 2.11ng/L

Detection range: 5-600ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 2.11ng/L

Detection range: 5-600ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Vascular Endothelial Cell Growth Factor A

Code

E0940Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

2.11ng/L

Detection range

5-600ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. May play a role in increasing vascular permeability during lactation, when increased transport of molecules from the blood is required for efficient milk protein synthesis. Binding to NRP1 receptor initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (By similarity).

UniProt accession

MASS(Da)

25,239

GeneID

83785

Synonyms

Vascular endothelial growth factor A;Vascular permeability factor;VEGF A;VEGFA;VEGF-A;VPF

Gene names

VEGFA

Research area

Cancer

Target protein

VEGFA

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0940Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
320ng/L 2.012 1.909 1.852
1.806
160ng/L 1.173 1.164 1.107
1.155
80ng/L 0.566 0.56 0.503
0.554
40ng/L 0.29 0.282 0.225
0.275
20ng/L 0.105 0.113 0.056
0.12
0ng/L 0.054 0.057 0
0.06

Precision

We measured random samples of E0940Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 204 6.85 3.4
Intra-Assay 2 18 143.9 7.31 5.1
Intra-Assay 3 18 77.2 3 3.9

Protocols

References (2)

The effects of ozone on bacterial growth and thiol-disulphide homeostasis in vascular graft infection caused by MRSA in rats
Molecular assessment, characterization, and differentiation of theca stem cells imply the presence of mesenchymal and pluripotent stem cells in sheep ovarian theca layer

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  • Size

    Code

    Price

  • 48T

    E0940Ra

    $320.00

  • 96T

    E0940Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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