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Home > Product > ELISA Kit > Rat Angiotensin Converting Enzyme 2, ACE2 ELISA Kit

Rat Angiotensin Converting Enzyme 2, ACE2 ELISA Kit

Rat Angiotensin Converting Enzyme 2, ACE2 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.23ng/ml

Detection range: 0.5-100ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 0.23ng/ml

Detection range: 0.5-100ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Angiotensin Converting Enzyme 2

Code

E0968Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.23ng/ml

Detection range

0.5-100ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Essential counter-regulatory carboxypeptidase of the renin-angiotensin hormone system that is a critical regulator of blood volume, systemic vascular resistance, and thus cardiovascular homeostasis. Converts angiotensin I to angiotensin 1-9, a nine-amino acid Peptide with anti-hypertrophic effects in cardiomyocytes, and angiotensin II to angiotensin 1-7, which then acts as a beneficial vasodilator and anti-proliferation agent, counterbalancing the actions of the vasoconstrictor angiotensin II. Also removes the C-terminal residue from three other vasoactive Peptides, neurotensin, kinetensin, and des-Arg bradykinin, but is not active on bradykinin. Also cleaves other biological Peptides, such as apelins, casomorphins and dynorphin A. Plays an important role in amino acid transport by acting as binding partner of amino acid transporter SLC6A19 in intestine, regulating trafficking, expression on the cell surface, and its catalytic activity.

UniProt accession

MASS(Da)

92,491

GeneID

302668

Synonyms

ACE 2;ACE2;ACE-related carboxypeptidase;Angiotensin-converting enzyme 2;Processed angiotensin-converting enzyme 2

Gene names

ACE2

Research area

Microbiology; Cell Biology; Cardiovascular

Target protein

ACE2

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of Rat Angiotensin Converting Enzyme 2, ACE2 ELISA Kit are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
64ng/ml 1.965 1.91 1.85
1.854
32ng/ml 0.918 0.972 0.913
1.027
16ng/ml 0.45 0.496 0.436
0.543
8ng/ml 0.239 0.246 0.186
0.252
4ng/ml 0.135 0.144 0.084
0.153
0ng/ml 0.055 0.06 0
0.064

Precision

We measured random samples of E0968Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 60.9 1.52 2.5
Intra-Assay 2 18 26.3 1.24 4.7
Intra-Assay 3 18 18.6 0.95 5.1

Protocols

References

Customer reviews and Q&As

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Product size

Price

  • 48T

    $320.00

  • 96T

    $458.00

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Lead time: Within one week

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