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Home > Product > ELISA Kit > Rat Alpha-Synuclein, SNCA ELISA Kit

Rat Alpha-Synuclein, SNCA ELISA Kit

Rat Alpha-Synuclein, SNCA ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.15ng/ml

Detection range: 0.5-100ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 0.15ng/ml

Detection range: 0.5-100ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Alpha-Synuclein

Code

E1113Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.15ng/ml

Detection range

0.5-100ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release. Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores. Mechanistically, acts by increasing local Ca(2+) release from microdomains which is essential for the enhancement of ATP-induced exocytosis. Acts also as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5. This chaperone activity is important to sustain normal SNARE-complex assembly during aging. Plays also a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity.

UniProt accession

MASS(Da)

14,515

GeneID

29219

Synonyms

Alpha-synuclein;SNCA

Gene names

SNCA

Research area

Neuroscience

Target protein

SNCA

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1113Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
64ng/ml 2.617 2.513 2.458
2.41
32ng/ml 1.505 1.456 1.401
1.408
16ng/ml 0.77 0.814 0.759
0.858
8ng/ml 0.471 0.436 0.381
0.402
4ng/ml 0.217 0.211 0.156
0.206
0ng/ml 0.05 0.055 0
0.06

Precision

We measured random samples of E1113Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 40.3 1.13 2.8
Intra-Assay 2 18 25 1.28 5.1
Intra-Assay 3 18 13.5 0.62 4.6

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E1113Ra

    $320.00

  • 96T

    E1113Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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