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Home > Product > ELISA Kit > Rat Calpain-1 Catalytic Subunit, CAPN1 ELISA Kit

Rat Calpain-1 Catalytic Subunit, CAPN1 ELISA Kit

Rat Calpain-1 Catalytic Subunit, CAPN1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.028ng/ml

Detection range: 0.05-30ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 0.028ng/ml

Detection range: 0.05-30ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Calpain-1 Catalytic Subunit

Code

E1324Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.028ng/ml

Detection range

0.05-30ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. Proteolytically cleaves CTBP1 at 'Asn-364', 'Gly-377' and 'His-399'.

UniProt accession

MASS(Da)

82,119

GeneID

29153

Synonyms

Calcium-activated neutral proteinase 1;Calpain mu-type;Calpain-1 catalytic subunit;Calpain-1 large subunit;CANP 1;CAPN 1;CAPN1;Micromolar-calpain;muCANP;

Gene names

CAPN1

Research area

Others

Target protein

CAPN1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1324Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
20ng/ml 2.351 2.222 2.181
2.093
10ng/ml 1.398 1.281 1.24
1.164
5ng/ml 0.64 0.731 0.69
0.823
2.5ng/ml 0.428 0.475 0.434
0.521
1.25ng/ml 0.227 0.203 0.162
0.179
0ng/ml 0.032 0.041 0
0.051

Precision

We measured random samples of E1324Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 6.8 0.43 6.4
Intra-Assay 2 18 14.3 0.52 3.6
Intra-Assay 3 18 3.8 0.24 6.2

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E1324Ra

    $320.00

  • 96T

    E1324Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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