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Home > Product > ELISA Kit > Rat Peroxiredoxin 4, PRDX4 ELISA Kit

Rat Peroxiredoxin 4, PRDX4 ELISA Kit

Rat Peroxiredoxin 4, PRDX4 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.051ng/ml

Detection range: 0.1-38ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 0.051ng/ml

Detection range: 0.1-38ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Peroxiredoxin 4

Code

E1634Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.051ng/ml

Detection range

0.1-38ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events. Regulates the activation of NF-kappa-B in the cytosol by a modulation of I-kappa-B-alpha phosphorylation.

UniProt accession

MASS(Da)

31,007

GeneID

85274

Synonyms

Antioxidant enzyme AOE372;Peroxiredoxin IV;Peroxiredoxin-4;PRDX 4;PRDX4;Prx-IV;Thioredoxin peroxidase AO372;Thioredoxin-dependent peroxide reductase A0372;Thioredoxin-dependent peroxiredoxin 4;

Gene names

PRDX4

Research area

Others

Target protein

PRDX4

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1634Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
20ng/ml 2.057 2.183 2.147
2.31
10ng/ml 1.74 1.699 1.662
1.657
5ng/ml 1.034 1.055 1.019
1.076
2.5ng/ml 0.637 0.669 0.632
0.701
1.25ng/ml 0.474 0.453 0.416
0.431
0ng/ml 0.044 0.036 0
0.028

Precision

We measured random samples of E1634Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 8.5 0.36 4.3
Intra-Assay 2 18 4.6 0.31 6.8
Intra-Assay 3 18 2 0.11 5.6

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E1634Ra

    $320.00

  • 96T

    E1634Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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