Rat Kininogen-1, KNG1 ELISA Kit

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Home > Product > ELISA Kit > Rat Kininogen-1, KNG1 ELISA Kit

Rat Kininogen-1, KNG1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 3.06ng/ml

Detection range: 5-1500ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 3.06ng/ml

Detection range: 5-1500ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Kininogen-1

Code

E1726Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

3.06ng/ml

Detection range

5-1500ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

(1) Kininogens are inhibitors of thiol proteases; (2) HMW-kininogen plays an important role in blood coagulation by helping to position optimally prekallikrein and factor XI next to factor XII; (3) HMW-kininogen inhibits the thrombin- and plasmin-induced aggregation of thrombocytes; (4) the active Peptide bradykinin that is released from HMW-kininogen shows a variety of physiological effects: (4A) influence in smooth muscle contraction, (4B) induction of hypotension, (4C) natriuresis and diuresis, (4D) decrease in blood glucose level, (4E) it is a mediator of inflammation and causes (4E1) increase in vascular permeability, (4E2) stimulation of nociceptors (4E3) release of other mediators of inflammation (e.g. prostaglandins), (4F) it has a cardioprotective effect (directly via bradykinin action, indirectly via endothelium-derived relaxing factor action); (5) LMW-kininogen inhibits the aggregation of thrombocytes; (6) LMW-kininogen is in contrast to HMW-kininogen not involved in blood clotting.

UniProt accession

P08934

MASS（Da）

70933

GeneID

25087

Synonyms

Bradykinin;Kininogen-1 Kininogen-1 heavy chain;Kininogen-1 light chain;KNG 1;KNG1

Gene names

KNG1

Research area

Cardiovascular

Target protein

KNG1

Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Protocol data

These standard curves of E1726Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
800ng/ml	1.718	1.794	1.757
	1.87
400ng/ml	0.948	0.97	0.933
	0.992
200ng/ml	0.42	0.504	0.467
	0.588
100ng/ml	0.27	0.262	0.226
	0.255
50ng/ml	0.138	0.109	0.073
	0.081
0ng/ml	0.039	0.037	0
	0.034

Precision

We measured random samples of E1726Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	404.8	17.95	4.4
Intra-Assay	2	18	578.6	17.95	3.1
Intra-Assay	3	18	226.3	10.52	4.6

Protocols

> Protocol Booklet

References

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Associated products

Datasheet

Size

Code

Price

48T

E1726Ra

$320.00
96T

E1726Ra

$458.00

Add to Cart

Lead time: Within two weeks

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