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Home > Product > ELISA Kit > Human Complement Factor B, CFB ELISA Kit

Human Complement Factor B, CFB ELISA Kit

Human Complement Factor B, CFB ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 4.19μg/ml

Detection range: 8-2000ug/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 96T,48T

Sensitivity: 4.19μg/ml

Detection range: 8-2000ug/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Complement Factor B

Code

E0379Hu

Assay type

Sandwich

Size

96T,48T

Sensitivity

4.19μg/ml

Detection range

8-2000ug/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Factor B which is part of the alternate pathway of the complement system is cleaved by factor D into 2 fragments: Ba and Bb. Bb, a serine protease, then combines with complement factor 3b to generate the C3 or C5 convertase. It has also been implicated in proliferation and differentiation of preactivated B-lymphocytes, rapid spreading of peripheral blood monocytes, stimulation of lymphocyte blastogenesis and lysis of erythrocytes. Ba inhibits the proliferation of preactivated B-lymphocytes.

UniProt accession

MASS(Da)

85,533

GeneID

629

Synonyms

C3/C5 convertase;CFB;Complement factor B;Complement factor B Ba fragment;Complement factor B Bb fragment;GBG;Glycine-rich beta glycoprotein;PBF2;Properdin factor B;

Gene names

CFB

Research area

Immunology

Target protein

CFB

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0379Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1200μg/ml 2.084 2.062 2.014
2.04
600μg/ml 0.917 0.997 0.95
1.078
300μg/ml 0.386 0.441 0.394
0.497
150μg/ml 0.202 0.224 0.177
0.247
75μg/ml 0.118 0.144 0.096
0.17
0μg/ml 0.033 0.048 0
0.062

Precision

We measured random samples of E0379Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 541.2 23.83 4.4
Intra-Assay 2 18 773.3 19.26 2.5
Intra-Assay 3 18 346.2 16.18 4.7

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 96T

    E0379Hu

    $458.00

  • 48T

    E0379Hu

    $320.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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