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Home > Product > ELISA Kit > Human Leukocyte Antigen C, HLA-C ELISA Kit

Human Leukocyte Antigen C, HLA-C ELISA Kit

Human Leukocyte Antigen C, HLA-C ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 0.2ng/ml

Detection range: 0.5-150ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 96T,48T

Sensitivity: 0.2ng/ml

Detection range: 0.5-150ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Leukocyte Antigen C

Code

E0436Hu

Assay type

Sandwich

Size

96T,48T

Sensitivity

0.2ng/ml

Detection range

0.5-150ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Antigen-presenting major histocompatibility complex class I (MHCI) molecule with an important role in reproduction and antiviral immunity (PubMed: 20972337, PubMed: 24091323, PubMed: 20439706, PubMed: 11172028, PubMed: 20104487, PubMed: 28649982, PubMed: 29312307). In complex with B2M/beta 2 microglobulin displays a restricted repertoire of self and viral Peptides and acts as a dominant ligand for inhibitory and activating killer immunoglobulin receptors (KIRs) expressed on NK cells (PubMed: 16141329). In an allogeneic setting, such as during pregnancy, mediates interaction of extravillous trophoblasts with KIR on uterine NK cells and regulate trophoblast invasion necessary for placentation and overall fetal growth (PubMed: 20972337, PubMed: 24091323). During viral infection, may present viral Peptides with low affinity for KIRs, impeding KIR-mediated inhibition through Peptide antagonism and favoring lysis of infected cells (PubMed: 20439706). Presents a restricted repertoire of viral Peptides on antigen-presenting cells for recognition by alpha-beta T cell receptor (TCR) on HLA-C-restricted CD8-positive T cells, guiding antigen-specific T cell immune response to eliminate infected cells, particularly in chronic viral infection settings such as HIV-1 or CMV infection (PubMed: 11172028, PubMed: 20104487, PubMed: 28649982). Both the Peptide and the MHC molecule are recognized by TCR, the Peptide is responsible for the fine specificity of antigen recognition and MHC residues account for the MHC restriction of T cells (By similarity). Typically presents intracellular Peptide antigens of 9 amino acids that arise from cytosolic proteolysis via proteasome. Can bind different Peptides containing allele-specific binding motifs, which are mainly defined by anchor residues at position 2 and 9. Preferentially displays Peptides having a restricted repertoire of hydrophobic or aromatic amino acids (Phe, Ile, Leu, Met, Val and Tyr) at the C-terminal anchor (PubMed: 8265661, PubMed: 25311805). ALLELE C*01:02: The Peptide-bound form interacts with KIR2DL2 and KIR2DL3 inhibitory receptors on NK cells. The low affinity Peptides compete with the high affinity Peptides impeding KIR-mediated inhibition and favoring lysis of infected cells (PubMed: 20439706). Presents to CD8-positive T cells a CMV epitope derived from UL83/pp65 (RCPEMISVL), an immediate-early antigen necessary for initiating viral replication (PubMed: 12947002). ALLELE C*04:01: Presents a conserved HIV-1 epitope derived from env (SFNCGGEFF) to memory CD8-positive T cells, eliciting very strong IFNG responses (PubMed: 20104487). Presents CMV epitope derived from UL83/pp65 (QYDPVAALF) to CD8-positive T cells, triggering T cell cytotoxic response (PubMed: 12947002). ALLELE C*05:01: Presents HIV-1 epitope derived from rev (SAEPVPLQL) to CD8-positive T cells, triggering T cell cytotoxic response. ALLELE C*06:02: In trophoblasts, interacts with KIR2DS2 on uterine NK cells and triggers NK cell activation, including secretion of cytokines such as GMCSF that enhances trophoblast migration. ALLELE C*07:02: Plays an important role in the control of chronic CMV infection. Presents immunodominant CMV epitopes derived from IE1 (LSEFCRVL and CRVLCCYVL) and UL28 (FRCPRRFCF), both antigens synthesized during immediate-early period of viral replication. Elicits a strong anti-viral CD8-positive T cell immune response that increases markedly with age. ALLELE C*08:01: Presents viral epitopes derived from CMV UL83 (VVCAHELVC) and IAV M1 (GILGFVFTL), triggering CD8-positive T cell cytotoxic response. ALLELE C*12:02: Presents CMV epitope derived from UL83 (VAFTSHEHF) to CD8-positive T cells. ALLELE C*15:02: Presents CMV epitope derived from UL83 CC (VVCAHELVC) to CD8-positive T cells, triggering T cell cytotoxic response.

UniProt accession

MASS(Da)

40,649

GeneID

3107

Synonyms

HLA C;HLA class I histocompatibility antigen, C alpha chain;HLAC;HLA-C;HLA-Cw;Human leukocyte antigen C

Gene names

HLA-C

Research area

Others

Target protein

HLA-C

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0436Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
80ng/ml 2.069 2.033 1.987
1.998
40ng/ml 1.071 1.073 1.027
1.075
20ng/ml 0.453 0.535 0.489
0.618
10ng/ml 0.245 0.262 0.216
0.28
5ng/ml 0.086 0.1 0.053
0.114
0ng/ml 0.033 0.046 0
0.06

Precision

We measured random samples of E0436Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 70.3 2.06 2.9
Intra-Assay 2 18 33.7 1.53 4.6
Intra-Assay 3 18 18.9 0.92 4.8

Protocols

References

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  • Size

    Code

    Price

  • 96T

    E0436Hu

    $458.00

  • 48T

    E0436Hu

    $320.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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