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Home > Product > ELISA Kit > Human Ankyrin B, ANK-B ELISA Kit

Human Ankyrin B, ANK-B ELISA Kit

Human Ankyrin B, ANK-B ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 0.018ng/mL

Detection range: 0.05-10ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 96T,48T

Sensitivity: 0.018ng/mL

Detection range: 0.05-10ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Ankyrin B

Code

E0662Hu

Assay type

Sandwich

Size

96T,48T

Sensitivity

0.018ng/mL

Detection range

0.05-10ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Plays an essential role in the localization and membrane stabilization of ion transporters and ion Channels in several cell types, including cardiomyocytes, as well as in striated muscle cells. In skeletal muscle, required for proper localization of DMD and DCTN4 and for the formation and/or stability of a special subset of microtubules associated with costameres and neuromuscular junctions. In cardiomyocytes, required for coordinate assembly of Na/Ca exchanger, SLC8A1/NCX1, Na/K ATPases ATP1A1 and ATP1A2 and inositol 1,4,5-trisphosphate (InsP3) receptors at sarcoplasmic reticulum/sarcolemma sites. Required for expression and targeting of SPTBN1 in neonatal cardiomyocytes and for the regulation of neonatal cardiomyocyte contraction rate (PubMed: 12571597). In the inner segment of rod photoreceptors, required for the coordinated expression of the Na/K ATPase, Na/Ca exchanger and beta-2-spectrin (SPTBN1) (By similarity). Plays a role in endocytosis and intracellular protein transport. Associates with phosphatidylinositol 3-phosphate (PI3P)-positive organelles and binds dynactin to promote long-range motility of cells. Recruits RABGAP1L to (PI3P)-positive early endosomes, where RABGAP1L inactivates RAB22A, and promotes polarized trafficking to the leading edge of the migrating cells. Part of the ANK2/RABGAP1L complex which is required for the polarized recycling of fibronectin receptor ITGA5 ITGB1 to the plasma membrane that enables continuous directional cell migration (By similarity).

UniProt accession

MASS(Da)

433,715

GeneID

287

Synonyms

ANK 2;ANK2;ANK-2;Ankyrin-2;Ankyrin-B;Brain ankyrin;Non-erythroid ankyrin

Gene names

ANK2

Research area

Others

Target protein

ANK2

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0662Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
6.4ng/mL 2.302 2.313 2.268
2.323
3.2ng/mL 1.874 1.901 1.856
1.928
1.6ng/mL 1.111 1.184 1.14
1.258
0.8ng/mL 0.671 0.637 0.592
0.603
0.4ng/mL 0.401 0.417 0.372
0.433
0ng/mL 0.037 0.045 0
0.053

Precision

We measured random samples of E0662Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 0.7 0.03 4.8
Intra-Assay 2 18 1.4 0.08 6
Intra-Assay 3 18 2.9 0.16 5.4

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 96T

    E0662Hu

    $458.00

  • 48T

    E0662Hu

    $320.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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