Tel:86 021 3100 7137

Publish and get free product : share with us your publications using BT LAB product and get free products.
Home > Product > ELISA Kit > Human Neutrophil Elastase, NE ELISA Kit

Human Neutrophil Elastase, NE ELISA Kit

Human Neutrophil Elastase, NE ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.251ng/ml

Detection range: 0.5-100ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.251ng/ml

Detection range: 0.5-100ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Neutrophil Elastase

Code

E0890Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.251ng/ml

Detection range

0.5-100ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis (PubMed: 15140022). Capable of killing E.coli but not S.aureus in vitro; digests outer membrane protein A (ompA) in E.coli and K.pneumoniae (PubMed: 10947984).

UniProt accession

MASS(Da)

28,518

GeneID

1991

Synonyms

Bone marrow serine protease;ELANE;Elastase-2;HLE;Human leukocyte elastase;Medullasin;Neutrophil elastase;PMN elastase

Gene names

ELANE

Research area

Signal Transduction

Target protein

ELANE

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0890Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
64ng/ml 2.279 2.321 2.277
2.362
32ng/ml 1.533 1.572 1.529
1.611
16ng/ml 1.175 1.137 1.093
1.098
8ng/ml 0.658 0.686 0.643
0.714
4ng/ml 0.41 0.41 0.366
0.41
0ng/ml 0.041 0.043 0
0.045

Precision

We measured random samples of E0890Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%

Protocols

References (1)

Sputum neutrophil elastase and its relation to pediatric bronchiectasis severity: A cross-sectional study

Customer reviews and Q&As

No reviews
No reviews
  • Size

    Code

    Price

  • 48T

    E0890Hu

    $320.00

  • 96T

    E0890Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

Newsletter

Sign up

Newsletter

Sign up