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Home > Product > ELISA Kit > Human Clusterin, CLU ELISA Kit

Human Clusterin, CLU ELISA Kit

Human Clusterin, CLU ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.31ng/ml

Detection range: 0.5-300ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.31ng/ml

Detection range: 0.5-300ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Clusterin

Code

E1189Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.31ng/ml

Detection range

0.5-300ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

[Isoform 1]: Functions as extracellular chaperone that prevents aggregation of non native proteins (PubMed: 11123922, PubMed: 19535339). Prevents stress-induced aggregation of blood plasma proteins (PubMed: 11123922, PubMed: 12176985, PubMed: 17260971, PubMed: 19996109). Inhibits formation of amyloid fibrils by APP, APOC2, B2M, CALCA, CSN3, SNCA and aggregation-prone LYZ variants (in vitro) (PubMed: 12047389, PubMed: 17412999, PubMed: 17407782). Does not require ATP (PubMed: 11123922). Maintains partially unfolded proteins in a state appropriate for subsequent refolding by other chaperones, such as HSPA8/HSC70 (PubMed: 11123922). Does not refold proteins by itself (PubMed: 11123922). Binding to cell surface receptors triggers internalization of the chaperone-client complex and subsequent lysosomal or proteasomal degradation (PubMed: 21505792). Protects cells against apoptosis and against cytolysis by complement (PubMed: 2780565). Intracellular forms interact with ubiquitin and SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes and promote the ubiquitination and subsequent proteasomal degradation of target proteins (PubMed: 20068069). Promotes proteasomal degradation of COMMD1 and IKBKB (PubMed: 20068069). Modulates NF-kappa-B transcriptional activity (PubMed: 12882985). A mitochondrial form suppresses BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis (PubMed: 16113678, PubMed: 17689225). Plays a role in the regulation of cell proliferation (PubMed: 19137541). An intracellular form suppresses stress-induced apoptosis by stabilizing mitochondrial membrane integrity through interaction with HSPA5 (PubMed: 22689054). Secreted form does not affect caspase or BAX-mediated intrinsic apoptosis and TNF-induced NF-kappa-B-activity (PubMed: 24073260). Secreted form act as an important modulator during neuronal differentiation through interaction with STMN3 (By similarity). Plays a role in the clearance of immune complexes that arise during cell injury (By similarity). [Isoform 6]: Does not affect caspase or BAX-mediated intrinsic apoptosis and TNF-induced NF-kappa-B-activity. [Isoform 4]: Does not affect caspase or BAX-mediated intrinsic apoptosis and TNF-induced NF-kappa-B-activity (PubMed: 24073260). Promotes cell death through interaction with BCL2L1 that releases and activates BAX (PubMed: 21567405).

UniProt accession

MASS(Da)

52,495

GeneID

1191

Synonyms

Aging-associated gene 4 protein;Apo-J;ApoJalpha;ApoJbeta;Apolipoprotein J;CLI;CLU;Clusterin;Clusterin alpha chain;Clusterin beta chain;Complement cytolysis inhibitor;Complement cytolysis inhibitor a chain;Complement cytolysis inhibitor b chain;Complement-associated protein SP-40,40;Ku70-binding protein 1;NA1/NA2;SGP-2;Sulfated glycoprotein 2;Testosterone-repressed prostate message 2;TRPM-2

Gene names

CLU

Research area

Cardiovascular

Target protein

CLU

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1189Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
160ng/ml 2.07 2.177 2.14
2.284
80ng/ml 1.68 1.624 1.587
1.567
40ng/ml 1.033 1.027 0.99
1.021
20ng/ml 0.733 0.757 0.72
0.781
10ng/ml 0.405 0.45 0.413
0.495
0ng/ml 0.041 0.037 0
0.033

Precision

We measured random samples of E1189Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%

Protocols

References

Clusterin serum level: how does it affect psoriatic patients?
Serum clusterin in psoriasis: relation to metabolic syndrome

Customer reviews and Q&As

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Product size

Price

  • 48T

    $320.00

  • 96T

    $458.00

Add to Cart

Lead time: Within one week

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