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Home > Product > ELISA Kit > Human Caveolin-1, CAV-1 ELISA Kit

Human Caveolin-1, CAV-1 ELISA Kit

Human Caveolin-1, CAV-1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.151ng/ml

Detection range: 0.3-90ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.151ng/ml

Detection range: 0.3-90ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Caveolin-1

Code

E1727Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.151ng/ml

Detection range

0.3-90ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

May act as a scaffolding protein within caveolar membranes (PubMed: 11751885). Forms a stable heterooligomeric complex with CAV2 that targets to lipid rafts and drives caveolae formation. Mediates the recruitment of CAVIN proteins (CAVIN1/2/3/4) to the caveolae (PubMed: 19262564). Interacts directly with G-protein alpha subunits and can functionally regulate their activity (By similarity). Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner (PubMed: 17287217). Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway (By similarity). Negatively regulates TGFB1-mediated activation of SMAD2/3 by mediating the internalization of TGFBR1 from membrane rafts leading to its subsequent degradation (PubMed: 25893292).

UniProt accession

MASS(Da)

20,472

GeneID

857

Synonyms

CAV 1;CAV1;Caveolin-1

Gene names

CAV1

Research area

Immunology

Target protein

CAV1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E1727Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
48ng/ml 1.968 2.03 1.982
2.093
24ng/ml 1.459 1.495 1.447
1.532
12ng/ml 0.665 0.664 0.616
0.663
6ng/ml 0.45 0.403 0.355
0.357
3ng/ml 0.182 0.194 0.146
0.206
0ng/ml 0.044 0.048 0
0.052

Precision

We measured random samples of E1727Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 20.9 0.58 2.8
Intra-Assay 2 18 16.8 0.76 4.5
Intra-Assay 3 18 8 0.3 3.7

Protocols

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  • Size

    Code

    Price

  • 48T

    E1727Hu

    $320.00

  • 96T

    E1727Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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