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Home > Product > ELISA Kit > Human Apelin, AP ELISA Kit

Human Apelin, AP ELISA Kit

Human Apelin, AP ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 3.47ng/L

Detection range: 7-1500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 3.47ng/L

Detection range: 7-1500ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Apelin

Code

E2014Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

3.47ng/L

Detection range

7-1500ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Endogenous ligand for the apelin receptor (APLNR) (PubMed: 10525157). Drives internalization of the apelin receptor (By similarity). Apelin-36 dissociates more hardly than (pyroglu)apelin-13 from APLNR (By similarity). Hormone involved in the regulation of cardiac precursor cell movements during gastrulation and heart morphogenesis (By similarity). Has an inhibitory effect on cytokine production in response to T-cell receptor/CD3 cross-linking; the oral intake of apelin in the colostrum and the milk might therefore modulate immune responses in neonates (By similarity). Plays a role in early coronary blood vessels formation (By similarity). Mediates myocardial contractility in an ERK1/2-dependent manner (By similarity). May also have a role in the central control of body fluid homeostasis by influencing vasopressin release and drinking behavior (By similarity). (Microbial infection) Endogenous ligand for the apelin receptor (APLNR), an alternative coreceptor with CD4 for HIV-1 infection (PubMed: 11090199). Inhibits HIV-1 entry in cells coexpressing CD4 and APLNR (PubMed: 11090199). Apelin-36 has a greater inhibitory activity on HIV infection than other synthetic apelin derivatives (PubMed: 11090199).

UniProt accession

MASS(Da)

8,569

GeneID

8862

Synonyms

Apelin;Apelin-13;Apelin-28;Apelin-31;Apelin-36;APJ endogenous ligand;APLN

Gene names

APLN

Research area

Neuroscience

Target protein

APLN

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2014Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
800ng/L 2.391 2.24 2.189
2.089
400ng/L 1.225 1.232 1.181
1.24
200ng/L 0.696 0.718 0.668
0.741
100ng/L 0.425 0.476 0.425
0.527
50ng/L 0.196 0.184 0.133
0.171
0ng/L 0.062 0.051 0
0.04

Precision

We measured random samples of E2014Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 146.5 9.62 6.6
Intra-Assay 2 18 330.5 21.58 6.5
Intra-Assay 3 18 440.9 12.67 2.9

Protocols

References (5)

Effects of synbiotic supplementation on metabolic parameters and apelin in women with polycystic ovary syndrome: a randomised double-blind placebo-controlled trial
Association of apelin levels in overweight-obese children with pubertal development, but not with insulin sensitivity: 6.5 years follow up evaluation
Serum omentin-1, vaspin, and apelin levels and central obesity in patients with nonalcoholic fatty liver disease
Correlation of serum apelin level with carotid intima-media thickness and insulin resistance in a sample of Egyptian patients with type 2 diabetes mellitus
Effects of synbiotic supplementation on metabolic parameters and apelin in women with polycystic ovary syndrome: a randomised double-blind placebo-controlled trial - Corrigendum

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  • Size

    Code

    Price

  • 48T

    E2014Hu

    $320.00

  • 96T

    E2014Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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