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Home > Product > ELISA Kit > Human Neuropilin-1, NRP1 ELISA Kit

Human Neuropilin-1, NRP1 ELISA Kit

Human Neuropilin-1, NRP1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.054ng/ml

Detection range: 0.1-40ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 0.054ng/ml

Detection range: 0.1-40ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Neuropilin-1

Code

E2101Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.054ng/ml

Detection range

0.1-40ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Cell-surface receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. Mediates the chemorepulsant activity of semaphorins (PubMed: 9288753, PubMed: 9529250, PubMed: 10688880). Recognizes a C-end rule (CendR) motif R/KXXR/K on its ligands which causes cellular internalization and vascular leakage (PubMed: 19805273). It binds to semaphorin 3A, the PLGF-2 isoform of PGF, the VEGF165 isoform of VEGFA and VEGFB (PubMed: 9288753, PubMed: 9529250, PubMed: 10688880, PubMed: 19805273). Coexpression with KDR results in increased VEGF165 binding to KDR as well as increased chemotaxis. Regulates VEGF-induced angiogenesis. Binding to VEGFA initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development (By similarity). Regulates mitochondrial iron transport via interaction with ABCB8/MITOSUR (PubMed: 30623799). [Isoform 2]: Binds VEGF-165 and may inhibit its binding to cells (PubMed: 10748121, PubMed: 26503042). May induce apoptosis by sequestering VEGF-165 (PubMed: 10748121). May bind as well various members of the semaphorin family. Its expression has an averse effect on blood vessel number and integrity. (Microbial infection) Acts as a host factor for human coronavirus SARS-CoV-2 infection. Recognizes and binds to CendR motif RRAR on SARS-CoV-2 spike protein S1 which enhances SARS-CoV-2 infection.

UniProt accession

MASS(Da)

103,134

GeneID

8829

Synonyms

CD antigen CD304;Neuropilin-1;NRP 1;NRP1;Vascular endothelial cell growth factor 165 receptor

Gene names

NRP1

Research area

Others

Target protein

NRP1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2101Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
24ng/ml 2.641 2.544 2.49
2.447
12ng/ml 1.479 1.475 1.421
1.471
6ng/ml 0.872 0.846 0.792
0.82
3ng/ml 0.521 0.476 0.422
0.431
1.5ng/ml 0.259 0.267 0.213
0.275
0ng/ml 0.063 0.054 0
0.045

Precision

We measured random samples of E2101Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 8.2 0.37 4.4
Intra-Assay 2 18 11.9 0.45 3.8
Intra-Assay 3 18 2.9 0.21 7.4

Protocols

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  • Size

    Code

    Price

  • 48T

    E2101Hu

    $320.00

  • 96T

    E2101Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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