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Home > Product > ELISA Kit > Human Atp-binding Cassette Sub-family A Member 2, ABCA2 ELISA Kit

Human Atp-binding Cassette Sub-family A Member 2, ABCA2 ELISA Kit

Human Atp-binding Cassette Sub-family A Member 2, ABCA2 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 4.57ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 4.57ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Atp-Binding Cassette Sub-Family A Member 2

Code

E2421Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

4.57ng/L

Detection range

10-2000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Probable lipid tranporter that modulates cholesterol sequestration in the late endosome/lysosome by regulating the intracellular sphingolipid metabolism, in turn participates in cholesterol homeostasis (PubMed: 15238223, PubMed: 21810484, PubMed: 24201375) (Probable). May alter the transbilayer distribution of ceramide in the intraluminal membrane lipid bilayer, favoring its retention in the outer leaflet that results in increased acid ceramidase activity in the late endosome/lysosome, facilitating ceramide deacylation to sphingosine leading to the sequestration of free cholesterol in lysosomes (PubMed: 24201375). In addition regulates amyloid-beta production either by activating a signaling pathway that regulates amyloid precursor protein transcription through the modulation of sphingolipid metabolism or through its role in gamma-secretase processing of APP (PubMed: 22086926, PubMed: 26510981). May play a role in myelin formation (By similarity).

UniProt accession

MASS(Da)

269,833

GeneID

20

Synonyms

ABCA 2;ABCA2;ATP-binding cassette 2;ATP-binding cassette sub-family A member 2;ATP-binding cassette transporter 2

Gene names

ABCA2

Research area

Others

Target protein

ABCA2

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of Human Atp-binding Cassette Sub-family A Member 2, ABCA2 ELISA Kit are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1200ng/L 2.249 2.16 2.116
2.071
600ng/L 1.81 1.752 1.708
1.694
300ng/L 1.169 1.183 1.139
1.197
150ng/L 0.679 0.702 0.658
0.725
75ng/L 0.44 0.4 0.356
0.36
0ng/L 0.054 0.044 0
0.034

Precision

We measured random samples of E2421Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 168.5 8.96 5.3
Intra-Assay 2 18 344.2 15.66 4.5
Intra-Assay 3 18 82.4 3.81 4.6

Protocols

References

Customer reviews and Q&As

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Product size

Price

  • 48T

    $320.00

  • 96T

    $458.00

Add to Cart

Lead time: Within one week

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