Tel:86 021 3100 7137

Publish and get free product : share with us your publications using BT LAB product and get free products.
Home > Product > ELISA Kit > Bovine Fibroblast Growth Factor 1, FGF1 ELISA Kit

Bovine Fibroblast Growth Factor 1, FGF1 ELISA Kit

Bovine Fibroblast Growth Factor 1, FGF1 ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 0.96ng/L

Detection range: 2-700ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Bovine

Product Summary

Size: 96T,48T

Sensitivity: 0.96ng/L

Detection range: 2-700ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Bovine

Product overview

Full product name

Fibroblast Growth Factor 1

Code

E2104Bo

Assay type

Sandwich

Size

96T,48T

Sensitivity

0.96ng/L

Detection range

2-700ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Bovine

Storage

2-8ºC

Assay time

1h 30m

Background

Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Acts as a ligand for FGFR1 and integrins. Binds to FGFR1 in the presence of heparin leading to FGFR1 dimerization and activation via sequential autophosphorylation on tyrosine residues which act as docking sites for interacting proteins, leading to the activation of several signaling cascades. Binds to integrin ITGAV:ITGB3. Its binding to integrin, subsequent ternary complex formation with integrin and FGFR1, and the recruitment of PTPN11 to the complex are essential for FGF1 signaling. Induces the phosphorylation and activation of FGFR1, FRS2, MAPK3/ERK1, MAPK1/ERK2 and AKT1. Can induce angiogenesis (By similarity).

UniProt accession

MASS(Da)

17,493

GeneID

281160

Synonyms

Acidic eye-derived growth factor II;Acidic fibroblast growth factor;aFGF;ECGF;EDGF II;Endothelial cell growth factor;Endothelial cell growth factor alpha;Endothelial cell growth factor beta;FGF 1;FGF1;FGF-1;Fibroblast growth factor 1;HBGF-1;Heparin-binding growth factor 1;Prostatropin

Gene names

FGF1

Research area

Cardiovascular; Signal Transduction; Cancer

Target protein

FGF1

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2104Bo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
400ng/L 2.596 2.572 2.528
2.549
200ng/L 1.334 1.335 1.29
1.337
100ng/L 0.844 0.87 0.825
0.897
50ng/L 0.42 0.426 0.381
0.431
25ng/L 0.248 0.249 0.204
0.25
0ng/L 0.042 0.045 0
0.047

Precision

We measured random samples of E2104Bo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 207.8 6.6 3.2
Intra-Assay 2 18 133.7 6.77 5.1
Intra-Assay 3 18 63.8 3.26 5.1

Protocols

References

Customer reviews and Q&As

No reviews
No reviews
  • Size

    Code

    Price

  • 96T

    E2104Bo

    $458.00

  • 48T

    E2104Bo

    $320.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

Newsletter

Sign up

Newsletter

Sign up