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Home > Product > ELISA Kit > Bovine Interleukin-12 Subunit Beta, IL12B ELISA Kit

Bovine Interleukin-12 Subunit Beta, IL12B ELISA Kit

Bovine Interleukin-12 Subunit Beta, IL12B ELISA Kit

Product Summary

Size: 96T,48T

Sensitivity: 5.31ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Bovine

Product Summary

Size: 96T,48T

Sensitivity: 5.31ng/L

Detection range: 10-2000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Bovine

Product overview

Full product name

Interleukin-12 Subunit Beta

Code

E2225Bo

Assay type

Sandwich

Size

96T,48T

Sensitivity

5.31ng/L

Detection range

10-2000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Bovine

Storage

2-8ºC

Assay time

1h 30m

Background

Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC. Associates with IL23A to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis (By similarity).

UniProt accession

MASS(Da)

37,050

GeneID

281857

Synonyms

CLMF p40;Cytotoxic lymphocyte maturation factor 40 kDa subunit;IL 12B;IL 12beta;IL-12 subunit p40;IL12B;IL-12B;Interleukin-12 subunit beta

Gene names

IL12B

Research area

Immunology

Target protein

IL12B

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E2225Bo are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1200ng/L 2.315 2.289 2.228
2.263
600ng/L 1.32 1.314 1.253
1.308
300ng/L 0.668 0.783 0.721
0.897
150ng/L 0.414 0.475 0.414
0.536
75ng/L 0.287 0.295 0.234
0.303
0ng/L 0.058 0.061 0
0.064

Precision

We measured random samples of E2225Bo within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 424.3 24.45 5.8
Intra-Assay 2 18 691.1 23.02 3.3
Intra-Assay 3 18 185.9 13.46 7.2

Protocols

References (1)

High Granulocyte-Macrophage Colony Stimulating Factor to Interleukin 10 Ratio and Marked Antioxidant Enzyme Activities Predominate in Symptomatic Cattle Naturally Infected with Candidatus Mycoplasma haemobos, Theileria orientalis, Theileria sinensis and Trypanosoma evansi

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  • Size

    Code

    Price

  • 96T

    E2225Bo

    $458.00

  • 48T

    E2225Bo

    $320.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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