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Home > Product > ELISA Kit > Human Growth Differentiation Factor 9, GDF9 ELISA Kit

Human Growth Differentiation Factor 9, GDF9 ELISA Kit

Human Growth Differentiation Factor 9, GDF9 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 5.39ng/L

Detection range: 10-4000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 48T,96T

Sensitivity: 5.39ng/L

Detection range: 10-4000ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Growth Differentiation Factor 9

Code

E3059Hu

Assay type

Sandwich

Size

48T,96T

Sensitivity

5.39ng/L

Detection range

10-4000ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8ºC

Assay time

1h 30m

Background

Required for ovarian folliculogenesis. Promotes primordial follicle development. Stimulates granulosa cell proliferation. Promotes cell transition from G0/G1 to S and G2/M phases, through an increase of CCND1 and CCNE1 expression, and RB1 phosphorylation. It regulates STAR expression and cAMP-dependent progesterone release in granulosa and thecal cells. Attenuates the suppressive effects of activin A on STAR expression and progesterone production by increasing the expression of inhibin B. It suppresses FST and FSTL3 production in granulosa-lutein cells.

UniProt accession

MASS(Da)

51,444

GeneID

2661

Synonyms

GDF 9;GDF9;GDF-9;Growth/differentiation factor 9

Gene names

GDF9

Research area

Cardiovascular

Target protein

GDF9

Components

Components Quantity
Pre-coated ELISA Plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E3059Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2400ng/L 2.238 2.177 2.144
2.115
1200ng/L 1.934 1.886 1.854
1.839
600ng/L 1.135 1.115 1.083
1.096
300ng/L 0.748 0.674 0.641
0.599
150ng/L 0.381 0.393 0.36
0.405
0ng/L 0.035 0.033 0
0.031

Precision

We measured random samples of E3059Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 183 9.09 5
Intra-Assay 2 18 902.1 45.8 5.1
Intra-Assay 3 18 498.8 29.25 5.9

Protocols

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  • Size

    Code

    Price

  • 48T

    E3059Hu

    $320.00

  • 96T

    E3059Hu

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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