Sheep Fibroblast Growth Factor 1, FGF1 ELISA Kit

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Blood coagulation
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Home > Product > ELISA Kit > Sheep Fibroblast Growth Factor 1, FGF1 ELISA Kit

Sheep Fibroblast Growth Factor 1, FGF1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 1.57ng/L

Detection range: 3-900ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Sheep

Product Summary

Size: 48T,96T

Sensitivity: 1.57ng/L

Detection range: 3-900ng/L

Sample type: Serum, plasma, cell culture supernates

Reactive with: Sheep

Product overview

Full product name

Fibroblast Growth Factor 1

Code

E0074Sh

Assay type

Sandwich

Size

48T,96T

Sensitivity

1.57ng/L

Detection range

3-900ng/L

Sample type

Serum, plasma, cell culture supernates

Species

Sheep

Storage

2-8ºC

Assay time

1h 30m

Background

Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro. Acts as a ligand for FGFR1 and integrins. Binds to FGFR1 in the presence of heparin leading to FGFR1 dimerization and activation via sequential autophosphorylation on tyrosine residues which act as docking sites for interacting proteins, leading to the activation of several signaling cascades. Binds to integrin ITGAV:ITGB3. Its binding to integrin, subsequent ternary complex formation with integrin and FGFR1, and the recruitment of PTPN11 to the complex are essential for FGF1 signaling. Induces the phosphorylation and activation of FGFR1, FRS2, MAPK3/ERK1, MAPK1/ERK2 and AKT1. Can induce angiogenesis.

UniProt accession

Q7M303

MASS（Da）

17,557

GeneID

101109353

Synonyms

Acidic fibroblast growth factor;aFGF;FGF1;FGF-1;Fibroblast growth factor 1;HBGF-1;Heparin-binding growth factor 1

Gene names

FGF1

Research area

Cardiovascular; Signal Transduction; Cancer

Target protein

FGF1

Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Protocol data

These standard curves of E0074Sh are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
480ng/L	2.121	2.224	2.178
	2.326
240ng/L	1.947	1.949	1.903
	1.951
120ng/L	1.106	1.112	1.067
	1.119
60ng/L	0.622	0.631	0.585
	0.639
30ng/L	0.387	0.414	0.368
	0.441
0ng/L	0.056	0.045	0
	0.035

Precision

We measured random samples of E0074Sh within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	131.79	5.4	4.1
Intra-Assay	2	18	52.21	2.4	4.59
Intra-Assay	3	18	117.49	6.06	5.16

Protocols

> Protocol Booklet

References

Customer reviews and Q&As

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Associated products

Datasheet

Size

Code

Price

48T

E0074Sh

$320.00
96T

E0074Sh

$458.00

Add to Cart

Lead time: Within two weeks

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