Product overview
Full product name
Code
Assay type
Size
Sensitivity
Detection range
Sample type
Species
Storage
Assay time
UniProt accession
MASS（Da）
GeneID
Synonyms
Gene names
Research area
Target protein
Components
| Components | Quantity |
|---|---|
| Pre-coated ELISA plate | 12 * 8 well strips x1 |
| Standard solution | 0.5ml x1 |
| Standard diluent | 3ml x1 |
| Streptavidin-HRP | 6ml x1 |
| Stop solution | 6ml x1 |
| Substrate solution A | 6ml x1 |
| Substrate solution B | 6ml x1 |
| Wash buffer concentrate (25x) | 20ml x1 |
| Biotinylated antibody | 1ml x1 |
Protocol data
These standard curves of E0077Cat are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
| Concentration | O.D. | Average | Corrected |
| 120ng/ml | 2.349 | 2.354 | 2.295 |
| 2.359 | |||
| 60ng/ml | 1.471 | 1.478 | 1.419 |
| 1.484 | |||
| 30ng/ml | 0.759 | 0.764 | 0.705 |
| 0.769 | |||
| 15ng/ml | 0.554 | 0.546 | 0.487 |
| 0.539 | |||
| 7.5ng/ml | 0.285 | 0.283 | 0.224 |
| 0.282 | |||
| 0ng/ml | 0.068 | 0.059 | 0 |
| 0.049 |
Precision
We measured random samples of E0077Cat within the same batch/lot to ensure the consistency of the kits' performances.
| Intra/Inter-Assay | Sample | n | Mean | Standard Deviation | CV% |
| Intra-Assay | 1 | 18 | 55.86 | 2.15 | 3.84 |
| Intra-Assay | 2 | 18 | 13.75 | 0.82 | 5.99 |
| Intra-Assay | 3 | 18 | 31.68 | 1.97 | 6.21 |
Protocols
References
Customer reviews and Q&As
