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Home > Product > ELISA Kit > Horse Fibronectin, FN1 ELISA Kit

Horse Fibronectin, FN1 ELISA Kit

Horse Fibronectin, FN1 ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 2.45ng/mL

Detection range: 5-1000ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Horse

Product Summary

Size: 48T,96T

Sensitivity: 2.45ng/mL

Detection range: 5-1000ng/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Horse

Product overview

Full product name

Fibronectin

Code

E0118HO

Assay type

Sandwich

Size

48T,96T

Sensitivity

2.45ng/mL

Detection range

5-1000ng/mL

Sample type

Serum, plasma, cell culture supernates

Species

Horse

Storage

2-8ºC

Assay time

1h 30m

Background

Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape (By similarity). Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts (By similarity). Binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling. Probably involved in matrix organization of cartilage.

UniProt accession

MASS(Da)

272,168

GeneID

100034189

Synonyms

Anastellin;Fibronectin;FN;FN1

Gene names

FN1

Research area

Others

Target protein

FN1

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0118HO are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
640ng/mL 2.194 2.111 2.065
2.028
320ng/mL 1.544 1.43 1.385
1.316
160ng/mL 0.932 0.943 0.898
0.954
80ng/mL 0.412 0.439 0.394
0.466
40ng/mL 0.275 0.263 0.217
0.251
0ng/mL 0.045 0.046 0
0.046

Precision

We measured random samples of E0118HO within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 74.86 4.77 6.37
Intra-Assay 2 18 186.79 12.94 6.93
Intra-Assay 3 18 338.9 13.24 3.91

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E0118HO

    $360.00

  • 96T

    E0118HO

    $498.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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