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Home > Product > ELISA Kit > Rat Beta-glucuronidase, BETA GD ELISA Kit

Rat Beta-glucuronidase, BETA GD ELISA Kit

Rat Beta-glucuronidase, BETA GD ELISA Kit

Product Summary

Size: 48T,96T

Sensitivity: 0.02ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 48T,96T

Sensitivity: 0.02ng/ml

Detection range: 0.05-20ng/ml

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

beta-glucuronidase

Code

E0495Ra

Assay type

Sandwich

Size

48T,96T

Sensitivity

0.02ng/ml

Detection range

0.05-20ng/ml

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8ºC

Assay time

1h 30m

Background

Beta-glucuronidases are members of the glycosidase family of enzymes that catalyze breakdown of complex carbohydrates. Human β-glucuronidase is a type of glucuronidase (a member of glycosidase Family 2) that catalyzes hydrolysis of β-D-glucuronic acid residues from the non-reducing end of mucopolysaccharides (also referred to as glycosaminoglycans) such as heparan sulfate. Human β-glucuronidase is located in the lysosome. In the gut, brush border β-glucuronidase converts conjugated bilirubin to the unconjugated form for reabsorption. Beta-glucuronidase is also present in breast milk, which contributes to neonatal jaundice. The protein is encoded by the GUSB gene in humans and by the uidA gene in bacteria.

UniProt accession

MASS(Da)

74,793

GeneID

24434

Synonyms

Ac2-223;betaGD;Beta-glucuronidase;Gus-s

Gene names

Gusb

Research area

Tags & Cell Markers; Signal Transduction

Target protein

betaGD

Components

Components Quantity
Pre-coated ELISA plate 12 * 8 well strips x1
Standard solution 0.5ml x1
Standard diluent 3ml x1
Streptavidin-HRP 6ml x1
Stop solution 6ml x1
Substrate solution A 6ml x1
Substrate solution B 6ml x1
Wash buffer concentrate (25x) 20ml x1
Biotinylated antibody 1ml x1

Protocol data

These standard curves of E0495Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
12ng/ml 2.028 1.999 1.954
1.97
6ng/ml 1.307 1.409 1.364
1.511
3ng/ml 0.699 0.721 0.676
0.743
1.5ng/ml 0.397 0.382 0.337
0.367
0.75ng/ml 0.171 0.154 0.109
0.136
0ng/ml 0.052 0.045 0
0.038

Precision

We measured random samples of E0495Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-Assay 1 18 3.71 0.19 5.04
Intra-Assay 2 18 8.67 0.49 5.6
Intra-Assay 3 18 2.16 0.1 4.8

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 48T

    E0495Ra

    $320.00

  • 96T

    E0495Ra

    $458.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Kit catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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