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Home > Product > ELISA Primacu > Human Cystatin-F, CST7 Primacu™ ELISA Kit

Human Cystatin-F, CST7 Primacu™ ELISA Kit

Human Cystatin-F, CST7 Primacu™ ELISA Kit

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 67.55 pg/mL

Detection range: 234.4-15000 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 67.55 pg/mL

Detection range: 234.4-15000 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Cystatin-F

Code

BPE034

Assay type

Sandwich

Size

5*96T,96T,15*96T

Sensitivity

67.55 pg/mL

Detection range

234.4-15000 pg/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8°C

Assay time

3.5h

Background

The cystatin superfamily members are important natural cysteine protease inhibitors present in a wide variety of organisms and are divided into three classes. Cystatin F, also known as leukocystatin and CMAP (Cystatin-like Metastasis-Associated Protein), is a type 2 cystatin and its expression is limited to hematopoietic cells, with the highest expression levels being observed in monocytes, dendritic cells, and certain types of T-cells. Furthermore, cystatin F mRNA becomes up-regulated during dendritic cell maturation, and thus suggests a specific role of cystatin F in immune regulation. Cystatin F is produced as a dimer, an inactive cathepsin inhibitor which is activated by chemical reduction. In addition, Cystatin F and its homologues have been observed expressing in various human cancer cell lines established from malignant tumors, and thus indicates a new diagnosis and prevention approach of certain human carcinomas metastasis.

UniProt accession

MASS(Da)

16454

GeneID

8530

Gene names

CST7

Research area

Cardiovascular,Cell biology,Neuroscience

Target protein

Cystatin F

Components

Components Quantity
Pre-coated ELISA Plate 12 × 8 well strips × 1
Standard Solution 0.2mL × 1 vail
Dilution Buffer 20mL × 1 vail
Stop Solution 6mL × 1 vail
Substrate Solution A 12mL × 1 vail
Substrate Solution B 12mL × 1 vail
Wash Buffer Concentrate (25x) 20mL × 1 vail
Detection Antibody Concentrate 0.6mL × 1 vail

Protocol data

These standard curves of BPE034 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
15000 pg/ml 2.512 2.594 2.5
2.675
7500 pg/ml 1.242 1.194 1.1
1.145
3750 pg/ml 0.656 0.641 0.547
0.625
1875 pg/ml 0.482 0.46 0.366
0.437
937.5 pg/ml 0.251 0.242 0.148
0.232
468.75 pg/ml 0.152 0.146 0.052
0.14
234.375 pg/ml 0.114 0.111 0.017
0.108
0 pg/ml 0.097 0.094 0
0.091

Precision

We measured random samples of BPE034 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 20 723 37.3 5.2
Intra-assay 2 20 2261 75.9 3.4
Intra-assay 3 20 9042 244 2.7
Inter-assay 1 20 721 64 8.9
Inter-assay 2 20 2249 230 10.2
Inter-assay 3 20 9075 861 9.5

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 5*96T

    BPE034

    $2320.00

  • 96T

    BPE034

    $580.00

  • 15*96T

    BPE034

    $6525.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Primacu catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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