Full product name

Code

Assay type

Size

Sensitivity

Detection range

Sample type

Species

Storage

Assay time

Background

UniProt accession

MASS（Da）

GeneID

Gene names

Research area

Target protein

Components	Quantity
Pre-coated ELISA Plate	12 × 8 well strips × 1
Standard Solution	0.2mL × 1 vail
Dilution Buffer	20mL × 1 vail
Stop Solution	6mL × 1 vail
Substrate Solution A	12mL × 1 vail
Substrate Solution B	12mL × 1 vail
Wash Buffer Concentrate (25x)	20mL × 1 vail
Detection Antibody Concentrate	0.6mL × 1 vail

These standard curves of BPE030 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1200 pg/ml	2.166	2.252	2.163
	2.338
600 pg/ml	1.361	1.29	1.201
	1.219
300 pg/ml	0.761	0.722	0.633
	0.684
150 pg/ml	0.435	0.416	0.327
	0.398
75 pg/ml	0.265	0.252	0.163
	0.239
37.5 pg/ml	0.163	0.174	0.085
	0.186
18.75 pg/ml	0.123	0.127	0.038
	0.132
0 pg/ml	0.091	0.089	0
	0.088

We measured random samples of BPE030 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	20	720	35.2	4.9
Intra-assay	2	20	2322	80.1	3.4
Intra-assay	3	20	6634	133	2
Inter-assay	1	20	711	28.5	4
Inter-assay	2	20	2325	64.5	2.8
Inter-assay	3	20	6685	152	2.3

> Protocol Booklet

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