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Home > Product > ELISA Primacu > Human Platelet Endothelial Cell Adhesion Molecule, PECAM1 Primacu™ ELISA Kit

Human Platelet Endothelial Cell Adhesion Molecule, PECAM1 Primacu™ ELISA Kit

Human Platelet Endothelial Cell Adhesion Molecule, PECAM1 Primacu™ ELISA Kit

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 8.15 pg/mL

Detection range: 18.75-12000 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 8.15 pg/mL

Detection range: 18.75-12000 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Platelet endothelial cell adhesion molecule

Code

BPE030

Assay type

Sandwich

Size

5*96T,96T,15*96T

Sensitivity

8.15 pg/mL

Detection range

18.75-12000 pg/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8°C

Assay time

3.5h

Background

The Cluster of Differentiation 31 (CD31) adhesion molecule, also known as platelet-endothelial cell adhesion molecule-1 (PECAM-1), is the only known member of the CAM family on platelets. CD31 protein is a 130-kDa transmembrane glycoprotein expressed by endothelial cells, platelets, monocytes, neutrophils, and certain T cell subsets. CD31 protein is also expressed in certain tumors, including epithelioid hemangioendothelioma, other vascular tumors, and histiocytic malignancies. CD31 plays a key role in removing aged neutrophils and tissue regeneration. CD31 protein mediates the homotypic or heterotypic cell adhesion by binding to itself or the leukocyte integrin αvβ3, and thus plays a role in neutrophil recruitment in inflammatory responses, transendothelial migration of leukocytes, as well as in cardiovascular development.

UniProt accession

MASS(Da)

82522

GeneID

5175

Gene names

PECAM1

Research area

Immunology,Cancer,Cardiovascular,Signal transduction,Stem cells

Target protein

CD31

Components

Components Quantity
Pre-coated ELISA Plate 12 × 8 well strips × 1
Standard Solution 0.2mL × 1 vail
Dilution Buffer 20mL × 1 vail
Stop Solution 6mL × 1 vail
Substrate Solution A 12mL × 1 vail
Substrate Solution B 12mL × 1 vail
Wash Buffer Concentrate (25x) 20mL × 1 vail
Detection Antibody Concentrate 0.6mL × 1 vail

Protocol data

These standard curves of BPE030 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1200 pg/ml 2.166 2.252 2.163
2.338
600 pg/ml 1.361 1.29 1.201
1.219
300 pg/ml 0.761 0.722 0.633
0.684
150 pg/ml 0.435 0.416 0.327
0.398
75 pg/ml 0.265 0.252 0.163
0.239
37.5 pg/ml 0.163 0.174 0.085
0.186
18.75 pg/ml 0.123 0.127 0.038
0.132
0 pg/ml 0.091 0.089 0
0.088

Precision

We measured random samples of BPE030 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 20 720 35.2 4.9
Intra-assay 2 20 2322 80.1 3.4
Intra-assay 3 20 6634 133 2
Inter-assay 1 20 711 28.5 4
Inter-assay 2 20 2325 64.5 2.8
Inter-assay 3 20 6685 152 2.3

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 5*96T

    BPE030

    $2040.00

  • 96T

    BPE030

    $480.00

  • 15*96T

    BPE030

    $5400.00

Add to Cart

Lead time: Within two weeks

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