Full product name

Code

Assay type

Size

Sensitivity

Detection range

Sample type

Species

Storage

Assay time

Background

UniProt accession

MASS（Da）

GeneID

Gene names

Research area

Target protein

Components	Quantity
Pre-coated ELISA Plate	12 × 8 well strips × 1
Standard Solution	0.2mL × 1 vail
Dilution Buffer	20mL × 1 vail
Stop Solution	6mL × 1 vail
Substrate Solution A	12mL × 1 vail
Substrate Solution B	12mL × 1 vail
Wash Buffer Concentrate (25x)	20mL × 1 vail
Detection Antibody Concentrate	0.6mL × 1 vail

These standard curves of BPE026 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
10000 pg/ml	2.535	2.485	2.364
	2.435
5000 pg/ml	1.324	1.289	1.168
	1.254
2500 pg/ml	0.604	0.644	0.523
	0.683
1250 pg/ml	0.406	0.391	0.27
	0.376
625 pg/ml	0.262	0.249	0.128
	0.236
312.5 pg/ml	0.172	0.183	0.062
	0.194
156.25 pg/ml	0.154	0.157	0.036
	0.16
0 pg/ml	0.116	0.121	0
	0.126

We measured random samples of BPE026 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	20	499	46.2	9.3
Intra-assay	2	20	1994	84.2	4.2
Intra-assay	3	20	7387	321	4.3
Inter-assay	1	20	495	30.1	6.1
Inter-assay	2	20	1991	88	4.4
Inter-assay	3	20	7469	362	4.9

> Protocol Booklet

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