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Home > Product > ELISA Primacu > Influenza A H9N2 Hemagglutinin Primacu™ ELISA Kit

Influenza A H9N2 Hemagglutinin Primacu™ ELISA Kit

Influenza A H9N2 Hemagglutinin Primacu™ ELISA Kit

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 69.33 pg/mL

Detection range: 156-10000 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with:

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 69.33 pg/mL

Detection range: 156-10000 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with:

Product overview

Full product name

Influenza A H9N2 Hemagglutinin / HA

Code

BPE189

Assay type

Sandwich

Size

5*96T,96T,15*96T

Sensitivity

69.33 pg/mL

Detection range

156-10000 pg/mL

Sample type

Serum, plasma, cell culture supernates

Storage

2-8°C

Assay time

3.5h

Background

The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.

Research area

Microbiology

Target protein

Influenza A H9N2 Hemagglutinin

Components

Components Quantity
Pre-coated ELISA Plate 12 × 8 well strips × 1
Standard Solution 0.2mL × 1 vail
Dilution Buffer 20mL × 1 vail
Stop Solution 6mL × 1 vail
Substrate Solution A 12mL × 1 vail
Substrate Solution B 12mL × 1 vail
Wash Buffer Concentrate (25x) 20mL × 1 vail
Detection Antibody Concentrate 0.6mL × 1 vail

Protocol data

These standard curves of BPE189 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
5000 pg/ml 2.716 2.706 2.609
2.697
2500 pg/ml 1.449 1.519 1.422
1.589
1250 pg/ml 0.838 0.818 0.721
0.798
625 pg/ml 0.472 0.466 0.369
0.46
312.5 pg/ml 0.274 0.278 0.181
0.282
156.25 pg/ml 0.192 0.185 0.088
0.178
78.125 pg/ml 0.146 0.137 0.04
0.128
0 pg/ml 0.091 0.097 0
0.103

Precision

We measured random samples of BPE189 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 20 309 18.4 5.9
Intra-assay 2 20 1067 60.1 5.6
Intra-assay 3 20 5336 233 4.4
Inter-assay 1 20 308 16.8 5.5
Inter-assay 2 20 1057 60.9 5.8
Inter-assay 3 20 5348 253 4.7

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 5*96T

    BPE189

    $2320.00

  • 96T

    BPE189

    $580.00

  • 15*96T

    BPE189

    $6525.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Primacu catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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