Full product name

Code

Assay type

Size

Sensitivity

Detection range

Sample type

Species

Storage

Assay time

Background

UniProt accession

MASS（Da）

GeneID

Gene names

Research area

Target protein

Components	Quantity
Pre-coated ELISA Plate	12 × 8 well strips × 1
Standard Solution	0.2mL × 1 vail
Dilution Buffer	20mL × 1 vail
Stop Solution	6mL × 1 vail
Substrate Solution A	12mL × 1 vail
Substrate Solution B	12mL × 1 vail
Wash Buffer Concentrate (25x)	20mL × 1 vail
Detection Antibody Concentrate	0.6mL × 1 vail

These standard curves of BPE326 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
10000 pg/ml	2.357	2.443	2.348
	2.529
5000 pg/ml	1.185	1.176	1.081
	1.167
2500 pg/ml	0.58	0.603	0.508
	0.626
1250 pg/ml	0.311	0.333	0.238
	0.355
625 pg/ml	0.197	0.211	0.116
	0.224
312.5 pg/ml	0.146	0.15	0.055
	0.154
156.25 pg/ml	0.114	0.117	0.022
	0.12
0 pg/ml	0.094	0.095	0
	0.096

We measured random samples of BPE326 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	20	1123	44.1	3.9
Intra-assay	2	20	2495	89.6	3.6
Intra-assay	3	20	5940	281	4.7
Inter-assay	1	20	1132	93.7	8.3
Inter-assay	2	20	2523	161	6.4
Inter-assay	3	20	5896	350	5.9

> Protocol Booklet

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