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Home > Product > ELISA Primacu > Ferret Tumor Necrosis Factor Alpha Primacu™ ELISA Kit

Ferret Tumor Necrosis Factor Alpha Primacu™ ELISA Kit

Ferret Tumor Necrosis Factor Alpha Primacu™ ELISA Kit

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 20.83 pg/mL

Detection range: 78.13-5000 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Ferret

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 20.83 pg/mL

Detection range: 78.13-5000 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Ferret

Product overview

Full product name

Tumor necrosis factor alpha

Code

BPE344

Assay type

Sandwich

Size

5*96T,96T,15*96T

Sensitivity

20.83 pg/mL

Detection range

78.13-5000 pg/mL

Sample type

Serum, plasma, cell culture supernates

Species

Ferret

Storage

2-8°C

Assay time

3.5h

Background

Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55/60) and TNF-R2 (TNF receptor type 2; CD120b; p75/80), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment

Research area

Cancer,Cardiovascular,Immunology,Metabolism,Microbiology,Neuroscience,Signal transduction

Target protein

TNF-alpha

Components

Components Quantity
Pre-coated ELISA Plate 12 × 8 well strips × 1
Standard Solution 0.2mL × 1 vail
Dilution Buffer 20mL × 1 vail
Stop Solution 6mL × 1 vail
Substrate Solution A 12mL × 1 vail
Substrate Solution B 12mL × 1 vail
Wash Buffer Concentrate (25x) 20mL × 1 vail
Detection Antibody Concentrate 0.6mL × 1 vail

Protocol data

These standard curves of BPE344 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
5000 pg/ml 2.462 2.367 2.252
2.272
2500 pg/ml 1.187 1.154 1.039
1.121
1250 pg/ml 0.549 0.556 0.441
0.562
625 pg/ml 0.294 0.305 0.19
0.315
312.5 pg/ml 0.197 0.187 0.072
0.177
156.25 pg/ml 0.157 0.147 0.032
0.137
78.125 pg/ml 0.134 0.129 0.014
0.124
0 pg/ml 0.107 0.115 0
0.123

Precision

We measured random samples of BPE344 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 20 469 18.2 3.9
Intra-assay 2 20 1234 41.5 3.4
Intra-assay 3 20 3010 143 4.8
Inter-assay 1 20 474 38.8 8.2
Inter-assay 2 20 1223 80.6 6.6
Inter-assay 3 20 2991 176 5.9

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 5*96T

    BPE344

    $2320.00

  • 96T

    BPE344

    $580.00

  • 15*96T

    BPE344

    $6525.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Primacu catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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