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Home > Product > ELISA Primacu > Influenza A H1N1 Nucleoprotein Primacu™ ELISA Kit

Influenza A H1N1 Nucleoprotein Primacu™ ELISA Kit

Influenza A H1N1 Nucleoprotein Primacu™ ELISA Kit

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 13.59 pg/mL

Detection range: 62.5-4000 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with:

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 13.59 pg/mL

Detection range: 62.5-4000 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with:

Product overview

Full product name

Influenza A H1N1 (A/California/07/2009) Nucleoprotein / NP

Code

BPE002

Assay type

Sandwich

Size

5*96T,96T,15*96T

Sensitivity

13.59 pg/mL

Detection range

62.5-4000 pg/mL

Sample type

Serum, plasma, cell culture supernates

Storage

2-8°C

Assay time

3.5h

Background

Influenza A viral nucleoprotein (NP) plays a critical role in virus replication and host adaptation. The influenza A virus nucleoprotein (NP) is an essential multifunctional protein that encapsidates the viral genome and functions as an adapter between the virus and the host cell machinery. NPs from all strains of influenza A viruses contain two nuclear localization signals (NLSs): a well-studied monopartite NLS1 and a less-characterized NLS2, thought to be bipartite. The nucleocapsid is a complex of the viral nucleoprotein, RNA, and several other viral proteins. The nucleoprotein forms large, RNA-bound, helical filaments and acts as a scaffold for additional viral proteins.

Research area

Microbiology

Target protein

Influenza A H1N1 NP

Components

Components Quantity
Pre-coated ELISA Plate 12 × 8 well strips × 1
Standard Solution 0.2mL × 1 vail
Dilution Buffer 20mL × 1 vail
Stop Solution 6mL × 1 vail
Substrate Solution A 12mL × 1 vail
Substrate Solution B 12mL × 1 vail
Wash Buffer Concentrate (25x) 20mL × 1 vail
Detection Antibody Concentrate 0.6mL × 1 vail

Protocol data

These standard curves of BPE002 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
4000 pg/ml 2.475 2.442 2.317
2.408
2000 pg/ml 1.302 1.307 1.182
1.312
1000 pg/ml 0.743 0.697 0.572
0.65
500 pg/ml 0.431 0.413 0.288
0.395
250 pg/ml 0.27 0.263 0.138
0.256
125 pg/ml 0.197 0.191 0.066
0.185
62.5 pg/ml 0.162 0.156 0.031
0.149
0 pg/ml 0.125 0.125 0
0.124

Precision

We measured random samples of BPE002 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 20 216 12.8 5.9
Intra-assay 2 20 983 36.2 3.7
Intra-assay 3 20 2658 116 4.4
Inter-assay 1 20 216 20.3 9.4
Inter-assay 2 20 979 48.1 4.9
Inter-assay 3 20 2645 125 4.7

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 5*96T

    BPE002

    $2320.00

  • 96T

    BPE002

    $580.00

  • 15*96T

    BPE002

    $6525.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Primacu catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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