Full product name

Code

Assay type

Size

Sensitivity

Detection range

Sample type

Species

Storage

Assay time

Background

UniProt accession

MASS（Da）

GeneID

Gene names

Research area

Target protein

Components	Quantity
Pre-coated ELISA Plate	12 × 8 well strips × 1
Standard Solution	0.2mL × 1 vail
Dilution Buffer	20mL × 1 vail
Stop Solution	6mL × 1 vail
Substrate Solution A	12mL × 1 vail
Substrate Solution B	12mL × 1 vail
Wash Buffer Concentrate (25x)	20mL × 1 vail
Detection Antibody Concentrate	0.6mL × 1 vail

These standard curves of BPE255 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
3000 pg/ml	2.311	2.395	2.27
	2.478
1500 pg/ml	1.469	1.421	1.296
	1.372
750 pg/ml	0.765	0.779	0.654
	0.792
375 pg/ml	0.492	0.461	0.336
	0.43
187.5 pg/ml	0.3	0.29	0.165
	0.28
93.75 pg/ml	0.192	0.2	0.075
	0.207
46.875 pg/ml	0.177	0.176	0.051
	0.175
0 pg/ml	0.133	0.125	0
	0.117

We measured random samples of BPE255 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	20	389	36.1	9.3
Intra-assay	2	20	972	88.6	9.1
Intra-assay	3	20	2261	108	4.8
Inter-assay	1	20	386	37.3	9.7
Inter-assay	2	20	967	81.5	8.4
Inter-assay	3	20	2237	164	7.3

> Protocol Booklet

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