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Home > Product > ELISA Primacu > Ferret T-Cell Surface Glycoprotein Cd4 Primacu™ ELISA Kit

Ferret T-Cell Surface Glycoprotein Cd4 Primacu™ ELISA Kit

Ferret T-Cell Surface Glycoprotein Cd4 Primacu™ ELISA Kit

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 8.37 pg/mL

Detection range: 46.88-3000 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Ferret

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 8.37 pg/mL

Detection range: 46.88-3000 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Ferret

Product overview

Full product name

T-cell surface glycoprotein CD4

Code

BPE345

Assay type

Sandwich

Size

5*96T,96T,15*96T

Sensitivity

8.37 pg/mL

Detection range

46.88-3000 pg/mL

Sample type

Serum, plasma, cell culture supernates

Species

Ferret

Storage

2-8°C

Assay time

3.5h

Background

T-cell surface glycoprotein CD4, is a single-pass type I membrane protein. CD4 contains three Ig-like C2-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. CD4 is a glycoprotein expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells. The CD4 surface determinant, previously associated as a phenotypic marker for helper/inducer subsets of T lymphocytes, has now been critically identified as the binding/entry protein for human immunodeficiency viruses (HIV). The human CD4 molecule is readily detectable on monocytes, T lymphocytes, and brain tissues. All human tissue sources of CD4 bind radiolabeled gp120 to the same relative degree; however, the murine homologous protein, L3T4, does not bind the HIV envelope protein. CD4 is a co-receptor that assists the T cell receptor (TCR) to activate its T cell following an interaction with an antigen-presenting cell. Using its portion that resides inside the T cell, CD4 amplifies the signal generated by the TCR. CD4 interacts directly with MHC class II molecules on the surface of the antigen-presenting cell via its extracellular domain. The CD4 molecule is currently the object of intense interest and investigation both because of its role in normal T-cell function, and because of its role in HIV infection. CD4 is a primary receptor used by HIV-1 to gain entry into host T cells. HIV infection leads to a progressive reduction of the number of T cells possessing CD4 receptors.Viral protein U (VpU) of HIV-1 plays an important role in downregulation of the main HIV-1 receptor CD4 from the surface of infected cells. Physical binding of VpU to newly synthesized CD4 in the endoplasmic reticulum is an early step in a pathway leading to proteasomal degradation of CD4. Amino acids in both helices found in the cytoplasmic region of VpU in membrane-mimicking detergent micelles experience chemical shift perturbations upon binding to CD4, whereas amino acids between the two helices and at the C-terminus of VpU show no or only small changes, respectively. Paramagnetic spin labels were attached at three sequence positions of a CD4 peptide comprising the transmembrane and cytosolic domains of the receptor. VpU binds to a membrane-proximal region in the cytoplasmic domain of CD4

Research area

Immunology,Stem cells,Signal transduction

Target protein

CD4

Components

Components Quantity
Pre-coated ELISA Plate 12 × 8 well strips × 1
Standard Solution 0.2mL × 1 vail
Dilution Buffer 20mL × 1 vail
Stop Solution 6mL × 1 vail
Substrate Solution A 12mL × 1 vail
Substrate Solution B 12mL × 1 vail
Wash Buffer Concentrate (25x) 20mL × 1 vail
Detection Antibody Concentrate 0.6mL × 1 vail

Protocol data

These standard curves of BPE345 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
3000 pg/ml 2.447 2.569 2.462
2.69
1500 pg/ml 1.363 1.414 1.307
1.464
750 pg/ml 0.713 0.738 0.631
0.762
375 pg/ml 0.464 0.438 0.331
0.412
187.5 pg/ml 0.26 0.263 0.156
0.265
93.75 pg/ml 0.19 0.179 0.072
0.167
46.875 pg/ml 0.139 0.135 0.028
0.13
0 pg/ml 0.108 0.107 0
0.105

Precision

We measured random samples of BPE345 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 20 141 3.6 2.6
Intra-assay 2 20 470 17.8 3.8
Intra-assay 3 20 1567 62.2 4
Inter-assay 1 20 142 11.4 8.1
Inter-assay 2 20 467 37.8 8.1
Inter-assay 3 20 1550 92.3 6

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 5*96T

    BPE345

    $2320.00

  • 96T

    BPE345

    $580.00

  • 15*96T

    BPE345

    $6525.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Primacu catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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