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Home > Product > ELISA Primacu > Human Dickkopf-Related Protein 1, DKK1 Primacu™ ELISA Kit

Human Dickkopf-Related Protein 1, DKK1 Primacu™ ELISA Kit

Human Dickkopf-Related Protein 1, DKK1 Primacu™ ELISA Kit

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 7.92 pg/mL

Detection range: 39.06-2500 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 7.92 pg/mL

Detection range: 39.06-2500 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Human

Product overview

Full product name

Dickkopf-related protein 1

Code

BPE370

Assay type

Sandwich

Size

5*96T,96T,15*96T

Sensitivity

7.92 pg/mL

Detection range

39.06-2500 pg/mL

Sample type

Serum, plasma, cell culture supernates

Species

Human

Storage

2-8°C

Assay time

3.5h

Background

Dickkopf (DKK) family proteins, consisting of DKK-1, DKK-2, DKK-3 and DKK-4, function as secreted Wnt antagonists by inhibiting Wnt coreceptors LRP5/6. DKK-1, DKK-2, and DKK-4 also bind cell surface Kremen-1 or Kremen-2 and promote the internalization of LRP5/6. Dickkopf related protein 1 (DKK-1) was initially identified as an inducer of head formation in Xenopus embryos. DKK-1 protein modulates Wnt signaling pathway during embryonic development. Increased levels of DKK-1 are found in the majority of lung cancers, esophageal squamous cell carcinomas, and hormone-resistant breast cancers, while DKK-1 expression is decreased in malignant melanoma and colorectal cancers.

UniProt accession

MASS(Da)

28672

GeneID

22943

Gene names

DKK1

Research area

Cardiovascular,Developmental biology,Immunology,Stem cells

Target protein

DKK1

Components

Components Quantity
Pre-coated ELISA Plate 12 × 8 well strips × 1
Standard Solution 0.2mL × 1 vail
Dilution Buffer 20mL × 1 vail
Stop Solution 6mL × 1 vail
Substrate Solution A 12mL × 1 vail
Substrate Solution B 12mL × 1 vail
Wash Buffer Concentrate (25x) 20mL × 1 vail
Detection Antibody Concentrate 0.6mL × 1 vail

Protocol data

These standard curves of BPE370 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
2500 pg/ml 2.509 2.574 2.453
2.638
1250 pg/ml 1.271 1.235 1.114
1.198
625 pg/ml 0.646 0.612 0.491
0.578
312.5 pg/ml 0.325 0.319 0.198
0.313
156.25 pg/ml 0.231 0.218 0.097
0.204
78.125 pg/ml 0.173 0.17 0.049
0.166
39.0625 pg/ml 0.156 0.148 0.027
0.139
0 pg/ml 0.128 0.121 0
0.113

Precision

We measured random samples of BPE370 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 20 161 14.9 9.3
Intra-assay 2 20 460 19.4 4.2
Intra-assay 3 20 1314 56.8 4.3
Inter-assay 1 20 162 10 6.2
Inter-assay 2 20 457 20.9 4.6
Inter-assay 3 20 1312 62.7 4.8

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 5*96T

    BPE370

    $2040.00

  • 96T

    BPE370

    $480.00

  • 15*96T

    BPE370

    $5400.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Primacu catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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