Full product name

Code

Assay type

Size

Sensitivity

Detection range

Sample type

Species

Storage

Assay time

UniProt accession

Research area

Target protein

Components	Quantity
Pre-coated ELISA Plate	12 × 8 well strips × 1
Standard Solution	0.2mL × 1 vail
Dilution Buffer	20mL × 1 vail
Stop Solution	6mL × 1 vail
Substrate Solution A	12mL × 1 vail
Substrate Solution B	12mL × 1 vail
Wash Buffer Concentrate (25x)	20mL × 1 vail
Detection Antibody Concentrate	0.6mL × 1 vail

These standard curves of BPE398 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2500 pg/ml	2.283	2.345	2.244
	2.408
1250 pg/ml	1.198	1.18	1.079
	1.163
625 pg/ml	0.668	0.635	0.534
	0.603
312.5 pg/ml	0.333	0.355	0.254
	0.378
156.25 pg/ml	0.239	0.224	0.123
	0.21
78.125 pg/ml	0.16	0.163	0.062
	0.167
39.0625 pg/ml	0.134	0.132	0.031
	0.131
0 pg/ml	0.1	0.101	0
	0.103

We measured random samples of BPE398 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-assay	1	20	160	5.9	3.7
Intra-assay	2	20	517	16	3.1
Intra-assay	3	20	1203	63.8	5.3
Inter-assay	1	20	161	13.3	8.3
Inter-assay	2	20	513	31.8	6.2
Inter-assay	3	20	1189	83.5	7

> Protocol Booklet

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