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Home > Product > ELISA Primacu > Rat Proprotein Convertase 9, Pcsk9 Primacu™ ELISA Kit

Rat Proprotein Convertase 9, Pcsk9 Primacu™ ELISA Kit

Rat Proprotein Convertase 9, Pcsk9 Primacu™ ELISA Kit

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 5.46 pg/mL

Detection range: 23.44-1500 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 5.46 pg/mL

Detection range: 23.44-1500 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Proprotein convertase subtilisin/kexin type 9

Code

BPE347

Assay type

Sandwich

Size

5*96T,96T,15*96T

Sensitivity

5.46 pg/mL

Detection range

23.44-1500 pg/mL

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8°C

Assay time

3.5h

Background

Proprotein convertase subtilisin/kexin type 9 (PCSK9), also known as NARC1 (neural apoptosis regulated convertase), which is a newly identified human secretory subtilase belonging to the proteinase K subfamily of the secretory subtilase family. PCSK9 protein is an enzyme which in humans is encoded by the PCSK9 gene with orthologs found across many species. It is expressed in neuroepithelioma, colon carcinoma, hepatic and pancreatic cell lines, and in Schwann cells. PCSK9 protein is highly expressed in the liver and regulates low density lipoprotein receptor (LDLR) protein levels. Inhibition of PCSK9 protein function is currently being explored as a means of lowering cholesterol levels. Thereby, PCSK9 protein is regarded as a new strategy to treat hypercholesterolemia. PCSK9 protein contributes to cholesterol homeostasis and may have a role in the differentiation of cortical neurons.

UniProt accession

MASS(Da)

74709

GeneID

298296

Synonyms

Narc1

Gene names

Pcsk9

Research area

Cardiovascular,Cell biology,Metabolism,Neuroscience,Stem cells,Signal transduction

Target protein

PCSK9

Components

Components Quantity
Pre-coated ELISA Plate 12 × 8 well strips × 1
Standard Solution 0.2mL × 1 vail
Dilution Buffer 20mL × 1 vail
Stop Solution 6mL × 1 vail
Substrate Solution A 12mL × 1 vail
Substrate Solution B 12mL × 1 vail
Wash Buffer Concentrate (25x) 20mL × 1 vail
Detection Antibody Concentrate 0.6mL × 1 vail

Protocol data

These standard curves of BPE347 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1500 pg/ml 1.98 2.075 1.972
2.17
750 pg/ml 1.229 1.168 1.065
1.107
375 pg/ml 0.651 0.664 0.561
0.677
187.5 pg/ml 0.401 0.394 0.291
0.386
93.75 pg/ml 0.248 0.257 0.154
0.265
46.875 pg/ml 0.184 0.177 0.074
0.169
23.4375 pg/ml 0.153 0.144 0.041
0.135
0 pg/ml 0.105 0.103 0
0.101

Precision

We measured random samples of BPE347 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 20 111 10.2 9.2
Intra-assay 2 20 277 27.4 9.9
Intra-assay 3 20 990 96 9.7
Inter-assay 1 20 112 12.6 11.2
Inter-assay 2 20 279 29.6 10.6
Inter-assay 3 20 1000 91.6 9.2

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 5*96T

    BPE347

    $2320.00

  • 96T

    BPE347

    $580.00

  • 15*96T

    BPE347

    $6525.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Primacu catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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