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Home > Product > ELISA Primacu > Rat Vascular Endothelial Growth Factor A, Vegfa Primacu™ ELISA Kit

Rat Vascular Endothelial Growth Factor A, Vegfa Primacu™ ELISA Kit

Rat Vascular Endothelial Growth Factor A, Vegfa Primacu™ ELISA Kit

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 8.78 pg/mL

Detection range: 23.44-1500 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product Summary

Size: 5*96T,96T,15*96T

Sensitivity: 8.78 pg/mL

Detection range: 23.44-1500 pg/mL

Sample type: Serum, plasma, cell culture supernates

Reactive with: Rat

Product overview

Full product name

Vascular endothelial growth factor A

Code

BPE348

Assay type

Sandwich

Size

5*96T,96T,15*96T

Sensitivity

8.78 pg/mL

Detection range

23.44-1500 pg/mL

Sample type

Serum, plasma, cell culture supernates

Species

Rat

Storage

2-8°C

Assay time

3.5h

Background

Vascular endothelial growth factor (VEGF), also known as vascular permeability factor (VPF) and VEGF-A, is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult. It is a member of the platelet-derived growth factor (PDGF)/vascular endothelial growth factor (VEGF) family and often exists as a disulfide-linked homodimer. VEGF-A protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, inhibiting apoptosis and tumor growth. VEGF-A protein is also a vasodilator that increases microvascular permeability, thus it was originally referred to as vascular permeability factor.

UniProt accession

MASS(Da)

25239

GeneID

83785

Synonyms

Vegf

Gene names

Vegfa

Research area

Cancer,Cardiovascular,Metabolism,Neuroscience,Signal transduction

Target protein

VEGF164

Components

Components Quantity
Pre-coated ELISA Plate 12 × 8 well strips × 1
Standard Solution 0.2mL × 1 vail
Dilution Buffer 20mL × 1 vail
Stop Solution 6mL × 1 vail
Substrate Solution A 12mL × 1 vail
Substrate Solution B 12mL × 1 vail
Wash Buffer Concentrate (25x) 20mL × 1 vail
Detection Antibody Concentrate 0.6mL × 1 vail

Protocol data

These standard curves of BPE348 are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration O.D. Average Corrected
1500 pg/ml 3.052 2.911 2.779
2.77
750 pg/ml 1.69 1.684 1.552
1.678
375 pg/ml 0.914 0.935 0.803
0.957
187.5 pg/ml 0.588 0.556 0.424
0.524
93.75 pg/ml 0.338 0.36 0.228
0.382
46.875 pg/ml 0.259 0.248 0.116
0.238
23.4375 pg/ml 0.183 0.19 0.058
0.197
0 pg/ml 0.125 0.132 0
0.139

Precision

We measured random samples of BPE348 within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay Sample n Mean Standard Deviation CV%
Intra-assay 1 20 188 19.2 10.2
Intra-assay 2 20 460 16.5 3.6
Intra-assay 3 20 1179 40.9 3.5
Inter-assay 1 20 187 17.6 9.4
Inter-assay 2 20 464 36.1 7.8
Inter-assay 3 20 1177 94.8 8.1

Protocols

References

Customer reviews and Q&As

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  • Size

    Code

    Price

  • 5*96T

    BPE348

    $2320.00

  • 96T

    BPE348

    $580.00

  • 15*96T

    BPE348

    $6525.00

Add to Cart

Lead time: Within two weeks

We are continuously updating our ELISA Primacu catalog with advanced verified products. If you can not download the datasheet please send your request of our latest datasheet.

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